Phospho egfr py1068

Manufactured by Cell Signaling Technology

Sourced in United States

Phospho-EGFR (pY1068) is an antibody product used for the detection and quantification of epidermal growth factor receptor (EGFR) phosphorylated at tyrosine 1068 in various experimental applications.

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Lab products found in correlation

Phospho erk1 2, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Sc 03, by Santa Cruz Biotechnology (1 mentions) α tubulin, by Merck Group (1 mentions) Phospho vegfr2, by Cell Signaling Technology (1 mentions) Vegfr2, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Phospho-EGFR (85 citations) EGFR pY1068 (9 citations) PY1068 (3 citations)

2 protocols using phospho egfr py1068

EGF-induced Erk and EGFR phosphorylation

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Keratinocytes were plated and prepared for lysate collection as described above. EGF was added to cells for 15 min, after which cells were lysed and collected as above. Western blotting was performed as above and blots were probed for phospho-Erk1/2 (Cell Signaling #4370), phospho-EGFR (pY1068) (Cell Signaling #3777), and β-actin (mouse monoclonal antibody; Cell Signaling #3700). Blots were imaged and analyzed as above.

Goglia A.G., Wilson M.Z., Jena S.G., Silbert J., Basta L.P., Devenport D, & Toettcher J.E. (2020). A live-cell screen for altered Erk dynamics reveals principles of proliferative control. Cell systems, 10(3), 240-253.e6.

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Immunoblot Analysis of Receptor Tyrosine Kinases

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Cell lysates were generated and immunoblots were performed as described previously.26 (link) Proteins were detected using antibodies against EGFR (SC-03; Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-EGFR pY1068 (Tyr1068; Cell Signaling, Danvers, MA, USA), α-Tubulin (Sigma-Aldrich Corp.), VEGFR2 (Cell Signaling), Phospho-VEGFR2 (Cell Signaling), horseradish peroxidase (HRP)-conjugated goat anti-mouse or goat anti-rabbit secondary antibody (Pierce; Rockford, IL, USA). Detected proteins were visualized by enhanced chemiluminescence using a Fotodyne imaging system (Hartland, WI, USA).

Rush J.S., Bingaman D.P., Chaney P.G., Wax M.B, & Ceresa B.P. (2016). Administration of Menadione, Vitamin K3, Ameliorates Off-Target Effects on Corneal Epithelial Wound Healing Due to Receptor Tyrosine Kinase Inhibition. Investigative Ophthalmology & Visual Science, 57(14), 5864-5871.

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