Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich, St. Louis, MA, USA) (with MOPS, pH 7.2), dimethylsulfoxide (DMSO), Sabouraud dextrose agar (SDA; Difco), saline solution (SS), iMarKTM microplate reader (Bio-Rad, Hercules, CA, USA), FLC, Bioscreen C equipment, Bioscreen software (Growth Curves USA, Piscataway, NJ, USA), 100-well microplates (honeycomb), LIVE/DEAD™ Yeast Viability kit (Thermo Fisher Scientific, Waltham, MA, USA), Olympus FV 1000 confocal microscope, Tescan Lyra 3 microscope, Fmoc-Arg(Pbf)–OH, Fmoc-Trp(Boc)–OH, Fmoc-Gln(Trt)–OH, Fmoc-Leu-OH, Fmoc-Lys(Fmoc)–OH, Fmoc-6-Ahx-OH, Rink amide resin, dicyclohexilcarbodiimide (DCC), and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). Trifluoroacetic acid (TFA), acetonitrile (ACN), dichloromethane (DCM), N,N-dimethylformamide, ethanodithiol, triisopropylsilane, methanol, acetonitrile, and isopropanol were obtained from Merck (Darmstadt, Germany). SPE SupelcleanTM columns were purchased from Sigma-Aldrich (St. Louis, MO, USA).
1 hydroxy 6 chlorobenzotriazole
Manufactured by AAPPTec
Sourced in United States
1-hydroxy-6-chlorobenzotriazole is a chemical reagent used in organic synthesis. It functions as a coupling agent and activator in peptide synthesis.
Automatically generated - may contain errors
Lab products found in correlation
Isopropanol, by Merck Group (5 mentions)
Acetonitrile, by Merck Group (5 mentions)
Methanol, by Merck Group (5 mentions)
Rink amide resin, by AAPPTec (4 mentions)
Triisopropylsilane, by Merck Group (3 mentions)
Fmoc trp boc oh, by AAPPTec (3 mentions)
Fmoc gln trt oh, by AAPPTec (3 mentions)
Fmoc leu oh, by AAPPTec (3 mentions)
Spe supelclean columns, by Merck Group (3 mentions)
N n dimethylformamide (dmf), by Merck Group (3 mentions)
Diisopropylethylamine, by Merck Group (3 mentions)
Ethanedithiol, by Merck Group (3 mentions)
Dichloromethane, by Merck Group (3 mentions)
Trifluoroacetic acid, by Merck Group (3 mentions)
1640 medium, by Merck Group (2 mentions)
Dicyclohexilcarbodiimide, by AAPPTec (2 mentions)
Ethanodithiol, by Merck Group (2 mentions)
Live dead yeast viability kit, by Thermo Fisher Scientific (2 mentions)
Imark microplate reader, by Bio-Rad (2 mentions)
Fmoc lys fmoc oh, by AAPPTec (2 mentions)
5 protocols using 1 hydroxy 6 chlorobenzotriazole
1
Antifungal Susceptibility Assay Protocol
2
Antifungal Susceptibility Assay Protocol
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Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich, St. Louis, MA, USA) (with MOPS, pH 7.2), dimethylsulfoxide (DMSO), Sabouraud dextrose agar (SDA; Difco), saline solution (SS), iMarKTM microplate reader (Bio-Rad, Hercules, CA, USA), FLC, Bioscreen C equipment, Bioscreen software (Growth Curves USA, Piscataway, NJ, USA), 100-well microplates (honeycomb), LIVE/DEAD™ Yeast Viability kit (Thermo Fisher Scientific, Waltham, MA, USA), Olympus FV 1000 confocal microscope, Tescan Lyra 3 microscope, Fmoc-Arg(Pbf)–OH, Fmoc-Trp(Boc)–OH, Fmoc-Gln(Trt)–OH, Fmoc-Leu-OH, Fmoc-Lys(Fmoc)–OH, Fmoc-6-Ahx-OH, Rink amide resin, dicyclohexilcarbodiimide (DCC), and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). Trifluoroacetic acid (TFA), acetonitrile (ACN), dichloromethane (DCM), N,N-dimethylformamide, ethanodithiol, triisopropylsilane, methanol, acetonitrile, and isopropanol were obtained from Merck (Darmstadt, Germany). SPE SupelcleanTM columns were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Solid-Phase Peptide Synthesis Reagents
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The Rink amide resin and Fmoc-Gly-OH, Fmoc-Ala-OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Cys(Trt)-OH, Fmoc-Met-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-His(Trt)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Gln(Trt)-OH, Fmoc-Pro-OH, Fmoc-Ahx, N,N-Dicyclohexylcarbodiimide, and 1-Hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). The reagents such as, acetonitrile, trifluoroacetic acid, dichloromethane, diisopropylethylamine, N, N-dimethylformamide, ethanedithiol, isopropanol, methanol, and triisopropylsilane were purchased from Merck (Darmstadt, Germany). SupelcleanTM SPE columns were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Silicycle® SiliaPrepTM C18 columns were kindly donated by EcoChem Especialidades Químicas (Waterloo, QC, Canada).
4
Peptide Synthesis and Cell Viability
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The HTB-132 MCF-7 and MCF-12 cell lines were obtained from ATCC® (Manassas, VA, USA); LIVE/DEADTM BacLightTM Viability Kit, and annexin V, Alexa FluorTM 488 conjugate was purchased from Invitrogen (Eugene, Oregon); Mitochondrial Membrane Potential Detection was obtained from BD Biosciences (Torreyana Rd., San Diego, CA 92121, USA). The DMEM culture medium was obtained from Sigma-Aldrich (St. Louis, MO, USA). Bovine fetal serum was purchased in Gibco (Waltham, MA, USA). The Fmoc-amino acids, Rink amide resin, dicyclohexylcarbodiimide and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). Acetonitrile, trifluoroacetic acid, dichloromethane, diisopropylethylamine, N, N-dimethylformamide, ethanedithiol, isopropanol, methanol, triisopropylsilane were purchased from Merck (Darmstadt, Germany). SPE SupelcleanTM columns were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Silicycle® SiliaPrepTM C18 columns were kindly donated by EcoChem Especialidades Químicas (Waterloo, QC, Canada).
5
Synthesis of Peptide-Based Compounds
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The resin Rink amide and the reagents Fmoc-Ser(tBu)-OH, Fmoc-Pro-OH, Fmoc-Ile-OH, Fmoc-Asn(Trt)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Lys(Boc)-OH, Fmoc-His(Trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Ala-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Cys(Trt)-OH, Fmoc-6-aminohexanoic acid (Fmoc-Ahx-OH), dicyclohexylcarbodiimide, and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). The reagents acetonitrile, trifluoroacetic acid, dichloromethane, diisopropylethylamine, N,N-dimethylformamide, ethanedithiol, isopropanol, methanol, and triisopropylsilane were purchased from Merck (Darmstadt, Germany). SPE columns Supelclean™, 6-maleimidohexanoic acid (6-MhxA), tetrabutylammonium chloride, glycidyl methacrylate (GMA), ethylene dimethacrylate (EDMA), cyclohexanol, dodecanol, 1,1′-azobis(cyclohexanecarbonitrile), silica gel, Tween 20, and 3,3′,5,5′-tetramethylbenzidine (Liquid Substrate System for ELISA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Potassium diacid phosphate, sodium acid phosphate, sodium chloride, and potassium chloride were purchased from AppliChem Panreac (Barcelona, Spain).
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