Agilent 1200 series dad detector

The EE was measured according to previously described approach [21 (link)]. Briefly, DS-M diluted 10 fold with distilled water was filtered through a 0.22 μm nylon (polyamide) membrane and 0.1 mL of the filtered liquid was accurately transferred into a volumetric flask containing 2 mL of methanol. After the structure of micelle was destroyed (ultrasound for 20 min), the sample was diluted to 5 mL with methanol and the DS containing in the micelle was determined using a HPLC method. The quantification of DS was performed using an Agilent 1100 series HPLC system (Agilent, Santa Clara, CA, USA), which was equipped with the Agilent 1200 series DAD detector and a reversed-phase C18 column (4.6 mm × 250 mm, 5 μm, Diamonsil plus). The data were captured and processed using Agilent ChemStation for LC 3D systems acquisition software. The mobile phase was a mixture of methanol, acetonitrile and water (pH = 3.0, 3:3:4, v/v) and eluted at a flow rate of 1.0 mL/min. Effluents were detected at 385 nm. This method has been validated for selectivity, linearity, limit of detection and quantification, accuracy, precision, as well as repeatability. The EE was determined using the following equation: