Lightcycler 480 2 instruments kit

During the outpatient visits, blood samples were drawn from the forearm vein with the subjects in the seated position. Analyses of serum albumin and APOE genes were performed in the Department of Laboratory Medicine in the hospital. Serum albumin levels were measured using a bromocresol green dye-binding assay (ADVIA 1800; Siemens, Washington, DC, USA). Since the included study subjects underwent multiple measurements of the serum albumin level over a long-term follow-up period, we calculated the median serum albumin levels, which were considered chronic serum albumin levels during the study period.
To determine APOE alleles, genomic DNA was extracted from the whole blood sample and subjected to APOE genotyping using the LightCycler 480 II Instruments Kit (Roche Diagnostics, Basel, Switzerland). APOE ε4 carriers were defined as the presence of at least one ε4 allele (ε2/4, ε3/4, ε4/4).

Genomic DNA was extracted from peripheral blood using QIAmp DNA Blood Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions. Human APOE C112R and R158C polymorphisms were detected to identify the APOE ε2, ε3, and ε4 alleles, using the LightCycler 480 II Instruments Kit (Roche Diagnostics, Basel, Switzerland) following manufacturer instructions.