Pcb 180

Once a day over the course of 15 days, the animals were exposed to PCB126 at 0.1, 1, or 10 μg/kg of body weight. Others groups of animals were exposed to PCB180 at 10 μg/kg of body weight. Control animals received only the vehicle solution of PCB126 or PCB180 that consisted of a 0.5% dimethyl sulfoxide (DMSO) saline solution. The exposure was done by nasal instillation (50 μL), and 5 hours after the last PCB126, PCB180 or vehicle exposure, the animals were anaesthetised and euthanised, and samples were collected. PCB126 (purity 99%) was purchased from AccuStandard (New Haven, CT, USA) and PCB180 (purity 99%) was purchased from Sigma-Aldrich (St Louis, USA). Schedule of PCB126 exposure was set up after previous dose/effect experiments, and the lower dose was chosen because it did not cause blood cell death but elevated the levels of PCB126 and AhR in the tissues of the lungs and liver (10 μg/kg). PCB180 dose was based on PCB126 dose, which caused alterations on GPCR pathways on leukocytes.

The following equipment and materials have been used in this study: carbon dioxide (Air Liquide, Liège, Belgium); helium (Air Products, Brussels, Belgium); acetonitrile (Biosolve, Valkenswaard, Netherlands); luminometer Orion II (BRS, Drogenbos, Belgium); adenosine triphosphate (ATP), Dulbecco's modified Eagle's medium (DMEM), DMEM without red phenol, and fetal bovine serum and trypsin (Fisher Bioblock Scientific, Tournai, Belgium); Focus gas chromatograph and Polaris Q mass spectrometer (Interscience, Louvain-la-Neuve, Belgium); charcoal, dextran, o,p′-DDD, p,p′-DDD, p,p′-DDE, o,p′-DDT, p,p′-DDT, 17β-estradiol, HCB, α-HCH, β-HCH, γ-HCH, δ-HCH, nonane, PCB 80 ¹³C, PCB 138 (2,2′,3,4,4′,5′-hexachlorobiphenyl), PCB 153 (2,2′,4,4′,5,5′-hexachlorobiphenyl), and PCB 180 (2,2′,3,4,4′,5,5′-heptachlorobiphenyl) (Sigma-Aldrich, Bornem, Belgium); D-luciferin (potassium salt) (Synchem OHG, Kassel, Germany); HT8 column (VWR, Leuven, Belgium).

The PCB analytical standards with a purity degree more than 99% (PCB 180, 2,2′,3,4,4′,5,5′‐heptachlorobiphenyl; PCB 138, 2,2′,3,4,4′,5′‐hexachlorobiphenyl; PCB 153, 2,2′,4,4′,5,5′‐hexachlorobiphenyl; PCB 101, 2,2′,4,5,5′‐Pentachlorobiphenyl; PCB 52, 2,2′,5,5′‐tetrachlorobiphenyl and PCB 28, 2,4,4′‐trichlorobiphenyl) were obtained from Sigma‐Aldrich (USA). Methylbenzene, acetonitrile, methyl alcohol, MgSO₄, toluene, primary secondary amine (PSA), and NaCl were obtained from Merck Co. (Germany).