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2 protocols using potassium bromide

1

Mung Bean Protein Extraction and Characterization

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Peeled mung bean was obtained from Zhanchuang Agricultural Technology Co. Ltd., (Shandong, China). Alcalase (176,743 U/g) was acquired from Novo Co. Ltd., (Beijing, China). 1-anilino-8-naphthalene sulfonate (ANS) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were from Sigma Co., Ltd. (St. Louis, MO USA). Tris-HCl buffer, sodium dodecyl sulphate (SDS), glycerol, bromophenol blue, 2-mercaptoethanol, Coomassie brilliant blue (R-250), trichloroacetic acid (TCA), potassium bromide, pyrogallol, salicylic acid, ferrous sulfate, potassium ferricyanide, ferric chloride, and ferrous chloride were from Beijing Solarbio Technology Co. Ltd. (Beijing, China). All chemicals used in this study were of analytical grade.
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2

Bioactive Compound Extraction from Hemerocallis citrina

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Hemerocallis citrina Borani was obtained from Qingyang, Gansu, China. Standards of glucose, mannose, fucose, rhamnose, xylose, galactose, arabinose, glucuronic acid, ribose, and galacturonic acid were purchased from Shanghai Yuanye Biotechnology (Shanghai, China). Absolute ethanol was purchased from Fuyu Fine Chemical (Tianjin, China). Trifluoroacetic acid, methanol, sodium hydroxide, hydrochloric acid, carbazole, 1-phenyl-3-methyl-5-pyrazolone, 3,5-dinitrosalicylic acid, sulfuric acid, Coomash bright blue, chloroform, potassium bromide, sodium azide, sodium acetate, anthranone, and other reagents were purchased from Solarbio Biotechnology (Beijing, China). ABTS and FRAP assay kits were purchased from Suzhou Keming Biotechnology (Suzhou, China). Distilled water was used in these experiments.
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