Mouse anti arl13b

Rabbit and rat polyclonal anti-EHBP1L1 antibodies were raised as previously described (11 (link)). The following additional primary antibodies were used: rabbit anti-pericentrin (ATLAS antibodies, #HPA016820); mouse anti-acetylated tubulin and anti-FLAG M2 (Sigma-Aldrich, #T7451 and #F1804); mouse anti-Arl13b (Abcam, #ab136648); rabbit anti-ß-actin, anti-Arl13b, anti-CD2AP, and mouse anti-GAPDH (Proteintech, #20536-1-AP, #17711-1-AP, #51046-1-AP, and #66004-1-Ig); mouse anti-CIN85 and anti-Arp2 (Santa Cruz Biotechnology, #sc-166862 and #sc-166103); rabbit anti-myosin Va (Cell signaling, #3402) and mouse anti-MBP (Developmental Studies Hybridoma Bank). The secondary antibodies Alexa Fluor 488 donkey anti-rabbit IgG, anti-mouse IgG, Alexa Fluor 568 donkey anti-rabbit IgG, and anti-mouse IgG were purchased from Thermo Fisher Scientific. Horseradish peroxidase–conjugated anti-rabbit IgG and anti-mouse IgG were purchased from Cell Signaling Technology.

The following primary antibodies were used: rabbit anti-Nogo-A/RTN4 (AHP1799, Biorad, Feldkirchen, Germany), mouse anti-TOM20 (Santa Cruz, CA, USA, sc-17764), mouse anti-SIGMA1R (Santa Cruz, sc-137075), rat anti-SIGMA1R (Merck, Darmstadt, Germany, ICR-SIG1R-A), mouse anti-ACSL4 (Santa Cruz, sc-365230), mouse anti-Myc (Cell Signaling, Danvers, MA, USA, 2276), rabbit anti-HA (Sigma-Aldrich, St. Louis, MO, USA, H6908), rat anti-HA (Roche, Basel, Switzerland, 3F10), rabbit anti-VLGR1, raised against the against the C-terminus of murine VLGR1 (amino acids 6198–6307) and previously characterized in [9 (link),14 (link)], rabbit anti-α-Tubulin (Abcam, Cambridge, UK, DM1A), rabbit anit-COX IV (NEB, Ipswich, MA, USA, 3E11), rabbit anti-GFAP (Dako Agilent, Santa Clara, CA, USA, Z0334), rat anti-RFP (Chromotek, Planegg, Germany, 5F8), mouse anti-CLIMP63, (Enzo Life Sciences, G1/296), goat anti-Pericentrin 2 (Santa Cruz, C-16), mouse anti-Arl13b (Abcam, Cambridge, UK, ab136648), goat anti-Centrin 2 [15 (link)]. Secondary antibodies conjugated Alexa 488, Alexa 555, Alexa 568 or Alexa 647 were purchased from Molecular Probes (Life Technologies, Darmstadt, Germany) or Rockland Inc. (Gilbertsville, PA, USA). Nuclear DNA was stained with DAPI (4′,6-diamidino-2-phenylindole, 1 mg/mL: Sigma-Aldrich).