High-glucose Dulbecco's modified Eagle's medium was obtained from HyClone Co (Logan, Utah). Fetal bovine serum, trypsin-Ethylene Diamine Tetraacetic Acid (0.05%), and phenol red were provided by Gibco Co, Ltd (Thermo Fisher Scientific, Cleveland, Ohio). Emodin and STC were purchased from Solarbio Science and Technology Co (Beijing, China). PrimeScript RT reagent and SYBR PremixEx Taq II (TliRNaseH Plus) were purchased from TaKaRa Biotechnology Co, Ltd (Dalian, China). RNAex Pro RNA reagent was obtained from Accurate Biology Co, Ltd (Changsha, China). A ROS Detection Assay Kit was purchased from AmyJet Scientific Inc Co, Ltd (Wuhan, China). An α-Amylase (α-AMS) Assay Kit and Gamma-Glutamylcysteine Synthetase (γ-GCS) Assay Kit were provided by the Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). A Micro Reduced Glutathione (GSH) Assay Kit was provided by Solarbio Science and Technology Co (Beijing, China). Rabbit anti-S100A9 was purchased from Cell Signaling Technology, Inc (Beverly, Mass). Rabbit anti-VNN1 was purchased from the Proteintech Group (Chicago, Ill). Goat antirabbit IgG H&L (Horseradish Peroxidase) was obtained from Abcam (Cambridge, UK). An Annexin V-FITC/PI Apoptosis Detection Kit was provided by Dalian Meilun Biotechnology Co, Ltd (Dalian, China).
Rnaex pro rna reagent
Manufactured by Accurate Biology
Sourced in China
RNAex Pro RNA reagent is a solution designed for the extraction and purification of RNA from various biological samples. It is a guanidinium-based reagent that effectively lyses cells and denatures RNases, allowing for the efficient recovery of high-quality RNA.
Automatically generated - may contain errors
Lab products found in correlation
Sybr premix ex taq 2 tli rnaseh plus, by Takara Bio (2 mentions)
Abi 7500 real time pcr system, by Thermo Fisher Scientific (2 mentions)
Phenol red, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Primescript rt reagent, by Takara Bio (1 mentions)
α amylase α ams assay kit, by Nanjing Jiancheng (1 mentions)
Goat antirabbit igg h l horseradish peroxidase, by Abcam (1 mentions)
Micro reduced glutathione gsh assay kit, by Solarbio (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Meilun (1 mentions)
Emodin, by Solarbio (1 mentions)
Evo m mlv rt mix kit, by Accurate Biology (1 mentions)
Sybr green premix pro taq hs qpcr kit, by Accurate Biology (1 mentions)
Quantstudio 3, by Thermo Fisher Scientific (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
4 protocols using rnaex pro rna reagent
1
Emodin and STC Modulate Oxidative Stress in Cells
2
Total RNA Extraction and qPCR Analysis
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RNAex Pro RNA reagent purchased from Accurate Biology in Hunan, China was utilized for the isolation and extraction of total cell RNA. Subsequently, the synthesis of cDNA was performed using an Evo M-MLV RT MIX kit, also purchased from Accurate Biology in Hunan, China. The mRNA expression level of various genes was quantified using a SYBR® green premix Pro Taq HS qPCR kit (Accurate Biology; Hunan, China) in an ABI 7500 real-time PCR system (Applied Biosystems; CA, USA). Table 1 displays the primer sequences.
3
qRT-PCR Analysis of Cultured Cells
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For qRT-PCR analysis, the total RNAs from cultured cells or tissue were isolated by RNAex Pro RNA reagent (Accurate Biotechnology (Hunan) Co., Ltd) and performed on the Applied Biosystems QuantStudio3. Primer sequences are listed in Supplementary Table 2 .
4
Quantitative Real-Time PCR Analysis
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Total RNA was isolated using RNAex Pro RNA reagent (Accurate Biology; Hunan, China) according to the manufacturer's instructions. RNA was then reverse-transcribed using the PrimeScript® RT reagent kit (TaKaRa, Dalian, China), and mRNA expression levels of different genes were quantified using SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa, Dalian, China) in an ABI 7500 Real-Time PCR System (Applied Biosystems; CA, USA). The primer sequences for the genes are listed in Supplementary Table S1 , and they were purchased from Sangon Biotech (Shanghai, China). All gene expression levels were normalized to β-actin, and the fold changes between the different groups were calculated using a standard curve for quantitative analysis.
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