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3 protocols using anhydrous iron 3 chloride fecl3

1

Curcumin-Iron-Zinc Composite Analysis

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All
of the chemicals used in this study were
of analytical grade and were used without any further refinement.
Curcumin, anhydrous iron(III) chloride (FeCl3), and Zn(CH3CO2)2·2H2O were bought
from Sigma-Aldrich. The appropriate solvent for the samples, methanol
was purchased from Merck. For various spectroscopic studies, Millipore
water and dimethyl sulfoxide (DMSO) from Spectrochem were used as
solvents.
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2

Coagulation Factors and DNA Isolation

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Human FXII, FXIIa, PK, α-kallikrein and plasmin were from Enzyme Research Laboratory (South Bend, IN). Human factor XIa was from Haematologic Technologies (Burlington, VT). Type I fibrillar collagen was from Chrono-Log (Havertown, PA). Anhydrous iron (III) chloride (FeCl3, molecular mass 160.20 Daltons) and delipidated bovine serum albumin (BSA) was from Sigma-Aldrich. Phosphatidylcholine:phosphatidylserine (PC/PS) vesicles were form Avanti Polar Lipids (Alabaster, Alabama). S-2366 (L-pyro-Glu-L-Pro-L-Arg-p-nitroanilide) and S2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroaniline dihydrochloride.) were from DiaPharma (West Chester, OH). Z-Gly-Gly-Arg-AMC was from Bachem (Torrance, CA). PTT A silica-based activated partial thromboplastin time (aPTT) reagent was from Diagnostic Stago (Parsippany, NJ). Human genomic DNA was isolated from blood leukocytes by conventional phenol:chloroform extraction.
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3

Synthesis of Conductive PVA Polymer

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Polyvinyl alcohol (PVA) (Mw 70 000, 99% hydrolyzed), pyrrole monomer (reagent grade 98%), anhydrous iron(iii) chloride (FeCl3), and sodium dodecyl sulfate (C12H25NaO4S) anionic surfactant were purchased from Sigma-Aldrich.
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