Milliplex multi analyte profiling human cytokine chemokine magnetic bead panel 29 plex kit

The serum concentration of 29 cytokines and chemokines were measured using the MILLIPLEX Multi-Analyte Profiling Human Cytokine/Chemokine Magnetic Bead Panel 29-plex kit (EMD Millipore, Darmstadt, Germany) and a 2-laser Bio-Plex 200 suspension array system coupled to a Bio-Plex Pro Wash Station (Bio-Rad, Hercules, CA, USA) and according to the provider instructions. Bead-fluorescence intensity readings for all the samples and standards were converted to the corresponding analyte concentrations using the Bio-Plex Manager software v6.2 (Bio-Rad).
The following analytes were measured at baseline and at the time of the development of persistent post-COVID-19 syndrome: interleukin 1-α (IL-1α), IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, interferon α-2 (IFNα2), IFNγ, tumour necrosis factor-α (TNF-α), TNF-β, monocyte chemoattractant protein 1 (MCP-1)/CCL2, macrophage inflammatory protein 1-α (MIP-1α)/CCL3, macrophage inflammatory protein-1β (MIP-1β)/CCL4, IFNγ-induced protein (IP-10)/CXCL10, eotaxin-1/CCL11, epidermal growth factor (EGF), granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and vascular endothelial growth factor (VEGF).