Cells were seeded in 96-well plates and treated with 25 μM erlotinib for 24 h. Intracellular ADP/ATP ratio was measured using a bioluminescent ADP/ATP Ratio Assay Kit according to the manufacturer’s instructions (Abcam, Cambridge, MA). Mitochondrial membrane depolarization was determined using the JC-1 fluorescence probe according to the manufacturer’s instructions (Molecular Probes). Cells were labeled with 2 μM JC-1 for 30 min at 37 °C in the dark and then analyzed at 488 nm excitation with 530/30 or 585/42 nm bypass emission filters using a fluorescence microplate reader (Bio-TeK Instruments).
Jc 1 fluorescence probe
Manufactured by Thermo Fisher Scientific
The JC-1 fluorescence probe is a dye used to measure the mitochondrial membrane potential in cells. It exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (529 nm) to red (590 nm). The ratio of red to green fluorescence intensity is commonly used as an indicator of the mitochondrial membrane potential.
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Lab products found in correlation
2 protocols using jc 1 fluorescence probe
1
Erlotinib-Induced Mitochondrial Dysfunction
2
Erlotinib-Induced Mitochondrial Dysfunction
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