Nucleosil silica

The AGP (from pooled human serum, 99% pure), periodic acid reagent (H₅IO₆), glycogen (bovine liver), HSA (Cohn fraction V, 99% globulin free, 99% fatty acid free), amitriptyline, carbamazepine, chloramphenicol, chlorpromazine, disopyramide, imipramine, lidocaine, nortriptyline, S-propranolol, and quinidine were from Sigma-Aldrich (St. Louis, MO, USA). The Nucleosil silica (7 μm particle diameter, 100 or 300 Å pore size) was obtained from Macherey Nagel (Düren, Germany). Reagents for the micro bicinchoninic (BCA) protein assay were from Pierce (Rockford, IL, USA). All other chemicals were of the purist grades available. All solutions were prepared using water from a Barnstead NANOpure system (Dubuque, IA, USA) or a Milli-Q Advantage A10 purification system (Millipore, Billerica, MA, USA) and were filtered through 0.20 μm GNWP nylon membranes from Millipore.

The following chemicals and materials were from Sigma-Aldrich (St Louis, MO, USA): AGP (from pooled human plasma, product G9885, ≥ 99% pure), L-fucose (> 99%), Con A (product C7275, type V, highly purified lyophilized powder), poly(ethylene oxide) (PEO; viscosity-average molecular mass, 8,000 kDa), Brij 35 (product 858366, number-average molecular mass, 1.198 kDa), Amicon Ultra centrifugal filter units (Ultra-4, 30 kDa cutoff), PNGase F (from Elizabethkingia meningoseptica, product P7367, ≥ 95%), iodoacetamide (IAA, ≥ 99%) and dithiothreitol (DTT, ≥ 98%). AAL (product L-1390, homogeneous by SDS-PAGE) was purchased from Vector Laboratories (Burlingame, CA, USA). Reagents for a micro bicinchoninic acid (BCA) protein assay were acquired from Thermo Fisher Scientific (Waltham, MA, USA). The NuTip HyperCarb PGC tips (1–10 μL) were from Glygen (Columbia, MD, USA), and the Nucleosil silica (300 Å pore size, 5 μm particle diameter) was from Macherey-Nagel (Duren, Germany). All aqueous solutions and samples were prepared using water purified by a Milli-Q Advantage A10 system (EMD Millipore, Billerica, MA, USA) and were filtered through 0.20 μm GNWP nylon membranes from Millipore.