Nucelospin rna plant and fungi kit

Manufactured by Macherey-Nagel

The NuceloSpin RNA Plant and Fungi kit is a product from Macherey-Nagel designed for the isolation of high-quality RNA from plant and fungal samples. The kit utilizes a silica-membrane technology to efficiently capture and purify RNA from various types of plant and fungal tissues.

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Lab products found in correlation

Revertaid rt reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Ssoadvanced universal sybr green supermix, by Bio-Rad (2 mentions) Cfx maestro software, by Bio-Rad (2 mentions) Cfx384 real time system, by Bio-Rad (1 mentions)

Close spelling variants of this product

NuceloSpin RNA kit RNA Plant kit

2 protocols using nucelospin rna plant and fungi kit

Quantitative Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fifteen-day-old
seedlings were transferred from solid half-strength MS plates to liquid
half-strength MS media (supplemented with 1% sucrose) for 5 days before
harvest and immediately frozen in liquid N₂. Total RNA
was extracted with NuceloSpin RNA Plant and Fungi kit (Macherey-Nagel).
cDNA was synthesized using RevertAid RT reverse transcription kit
(Thermo Fisher). Quantitative PCR reactions were conducted with the
CFX384 real-time system (Bio-Rad) and the SsoAdvanced Universal SYBR
Green Supermix (Bio-Rad) using the primers listed in Table S2. TIP41-like and PP2AA3 were used as reference genes to
normalize relative expression levels of all tested genes. Relative
expression was calculated using the CFX Maestro software (Bio-Rad).

Gaugler P., Schneider R., Liu G., Qiu D., Weber J., Schmid J., Jork N., Häner M., Ritter K., Fernández-Rebollo N., Giehl R.F., Trung M.N., Yadav R., Fiedler D., Gaugler V., Jessen H.J., Schaaf G, & Laha D. (2022). Arabidopsis PFA-DSP-Type Phosphohydrolases Target Specific Inositol Pyrophosphate Messengers. Biochemistry, 61(12), 1213-1227.

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Quantification of Gene Expression in Arabidopsis Seedlings

Check if the same lab product or an alternative is used in the 5 most similar protocols

15-days old seedlings were transferred from solid half-strength MS plates to liquid halfstrength MS media (supplemented with 1 % sucrose) for 5 days before harvest and immediately frozen in liquid N2. Total RNA was extracted with NuceloSpin RNA Plant and Fungi kit (Macherey-Nagel). cDNA was synthesized using RevertAid RT reverse transcription kit (Thermo Fisher). Quantitative PCR reactions were conducted with the CFX384 real-time system (Bio-Rad) and the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) using the primers listed in Table S1. TIP41like and PP2AA3 were used as reference genes to normalize relative expression levels of all tested genes. Relative expression was calculated using the CFX Maestro software (Bio-Rad).

Gaugler P., Schneider R., Liu G., Qiu D., Weber J., Schmid J., Jork N., Häner M., Ritter K., Fernández-Rebollo N., Giehl R.F.H., Trung M.N., Yadav R., Fiedler D., Gaugler V., Jessen H.J., Schaaf G., & Laha D. (2022). Arabidopsis PFA-DSP-type phosphohydrolases target specific inositol pyrophosphate messengers.

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