Western blot analyses were performed as previously described [14] . Briefly, cells were washed and scraped in a lysis buffer and quantified. Twenty µg of protein were loaded on a SDS–PAGE and transferred onto a 0.2 µm nitrocellulose membrane. Following blocking for 1 h with 5% non-fat dry milk in Tris-buffered saline with 1% tween-20 (TBS-T), membranes were incubated with the primary antibody overnight. After washing, the membrane was incubated for 1 h with the HRP-conjugated secondary antibody. Membranes were washed 3 times with TBS-T again, and proteins were visualized with Super Signal (Pierce, Bonn, Germany) enhanced chemiluminescence. Antibody for RAP1A (sc-1482) was from Santa Cruz (Heidelberg, Germany) and the RAS (610001) antibody was from BD Biosciences (Heidelberg, Germany). Antibodies for FAK and phosphoFAK (Tyr397) were from Cell Signaling Technology (Boston, USA).
Ras 610001 antibody
Manufactured by BD
Sourced in Germany
The RAS (610001) antibody is a laboratory reagent used in research applications. It is a monoclonal antibody that specifically binds to the RAS protein, which is a family of small GTPase proteins involved in cellular signal transduction pathways. The antibody can be used in various immunoassay techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the RAS protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Antibody for rap1a sc 1482, by Santa Cruz Biotechnology (2 mentions)
Farnesyl pyrophosphate, by Merck Group (1 mentions)
Geranylgeranyl pyrophosphate, by Merck Group (1 mentions)
Rho rac cdc42 activator 1, by Cytoskeleton (1 mentions)
Ml141, by Bio-Techne (1 mentions)
Fti 277, by Merck Group (1 mentions)
Mevalonate, by Merck Group (1 mentions)
Atorvastatin, by Merck Group (1 mentions)
Recombinant human dkk 1, by Thermo Fisher Scientific (1 mentions)
Ggti 298, by Merck Group (1 mentions)
Dharmafect, by Thermo Fisher Scientific (1 mentions)
Zoledronic acid, by Novartis (1 mentions)
2 protocols using ras 610001 antibody
1
Western Blot Analysis of Cellular Proteins
2
Regulation of RhoGTPases in Osteoblasts
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Zoledronic acid was provided by Novartis (Basel, Switzerland), and atorvastatin, mevalonate, geranyl-geranyl-pyrophosphate (GGPP), farnesyl pyrophosphate (FPP), GGTI-298 and FTI-277 were obtained from Sigma-Aldrich (Munich, Germany). Rac1 inhibitors #1 (553502) and #2 (553511), Y-27632, rho kinase inhibitor (H-1152P) and Clostridium difficile toxin A were from Merck Chemicals (Darmstadt, Germany). Cdc42 inhibitor ML-141 was from Tocris Bioscience (Bristol, UK). Rho inhibitor #2 (BML-EI394) was obtained from Enzo (Lörrach, Germany). Rho inhibitor #1 (CT04) and Rho/Rac/Cdc42 activator I were from Cytoskeleton Inc. (Denver, CO, USA). Recombinant human DKK-1 was obtained from Peprotech (Hamburg, Germany). Antibody for RAP1A (sc-1482) was from Santa Cruz (Heidelberg, Germany), RAS (610001) antibody was from BD Biosciences (Heidelberg, Germany), the DKK-1 (MAB10962) antibody was from R&D Systems (Wiesbaden, Germany) and all other antibodies were obtained from Cell Signaling Technology (Frankfurt, Germany). DKK-1 small interfering RNA (siRNA; s22721 and s22723), Cdc42 siRNAs and nontarget siRNA were purchased from Applied Biosystems (Darmstadt, Germany). Cells were transfected using Dharmafect (Thermo Scientific, Waltham, Massachusetts (MA) USA).
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