We monitored the mRNA expression levels of 24 mRNA transcripts, using TaqMan Low-Density array (TLDA) cards from Applied Biosystems (UK). Eighteen of the transcripts were analyzed in both studies (Additional file 1 ; overview of mRNA transcripts). mRNA transcripts were selected based on a thorough literature search investigating the effect of acute exercise on gene expression levels in PBMCs [36 (link)] as well as skeletal muscle [37 (link)]. Moreover, the selection was based on studies where effects of dairy products on markers of inflammation were investigated [38 (link)]. TLDA cards were used on a 7900 HT Applied Biosystems RT-qPCR machine (Applied Biosystems, UK). The Ct values were analyzed using SDS 2.4 (Applied Biosystems, UK), and further transferred to ExpressionSuite Sofware v1.0.3 (Applied Biosystems, UK). We normalized the Ct values to TATA box-binding protein (TBP) mRNA transcripts. Fold changes in mRNA transcripts from baseline to after the exercise session were calculated by dividing 2−ΔCtpost exercise with 2−ΔCtbaseline, using the 2−ΔΔCt-method [39 (link)].
Expressionsuite sofware v1
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
ExpressionSuite Software v1.0.3 is a data analysis software tool designed for gene expression studies. The software provides functionality for processing, analyzing, and visualizing gene expression data obtained from various experimental platforms.
Automatically generated - may contain errors
2 protocols using expressionsuite sofware v1
1
Transcriptional Response to Acute Exercise
2
Quantifying Gene Expression Changes
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We analyzed mRNA levels of 48 genes using TaqMan Low-Density array (TLDA) cards from Applied Biosystems, UK (see supplementary table 1 for an overview of all genes analyzed).
TLDA cards were run on a 7900 HT Applied Biosystems RT-qPCR machine (Applied Biosystems, UK). The cycle threshold values (Ct-values) were determined using SDS 2.4 (Applied Biosystems) and ExpressionSuite Sofware v1.0.3 (Applied Biosystems, UK). We normalized the Ct-values to TATA box binding protein (TBP) and beta-2-microglobulin (B2M) mRNA transcripts, and manually calculated relative changes in mRNA expression levels at baseline and at the end of the intervention (2 -ΔCt ). Fold changes in mRNA transcripts from baseline to end of intervention were calculated by dividing 2 -ΔCtend with 2 -ΔCtbaseline , using the 2 -ΔΔCt -method (42) .
TLDA cards were run on a 7900 HT Applied Biosystems RT-qPCR machine (Applied Biosystems, UK). The cycle threshold values (Ct-values) were determined using SDS 2.4 (Applied Biosystems) and ExpressionSuite Sofware v1.0.3 (Applied Biosystems, UK). We normalized the Ct-values to TATA box binding protein (TBP) and beta-2-microglobulin (B2M) mRNA transcripts, and manually calculated relative changes in mRNA expression levels at baseline and at the end of the intervention (2 -ΔCt ). Fold changes in mRNA transcripts from baseline to end of intervention were calculated by dividing 2 -ΔCtend with 2 -ΔCtbaseline , using the 2 -ΔΔCt -method (42) .
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