Bisbenzimide hepes

UV polymerizable NOA63 (Norland Optic Adhesives), PU (MINS-311RM, Minuta Tech.), and PET film (MITSUBISHI, Japan) were purchased and used without further purification. GoTaq^®DNA polymerase (M3005) was from Promega. E. coli O157:H7 (ATCC 43894) was obtained from ATCC. QuickExtract™ DNA extraction solution 1.0 (QE09050) was purchased from Epicentre. 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride hydrate, bisBenzimide (Hoechst 33258), and 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride hydrate, bisBenzimide (HEPES) were obtained from Sigma-Aldrich. The thermal cycler (C1000Touch™) device was obtained from Bio-Rad. UV visualizer (LIAS Slite 140) was obtained from Avegene Life Sciences. The DNA extraction and purification solution (QIAamp DNA mini kit, 51304) was purchased from QIAGEN. The electrochemical evaluation was carried out with the 630B (CH Instruments). The electron beam evaporator (EBS400) was purchased from EVATEC.

GoTaq^®DNA polymerase (M3005) was purchased from Promega (Madison, WI, USA). Bacillus cereus (ATCC 21768) were purchased from ATCC. 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride hydrate and 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride hydrate, bisBenzimide (HEPES) were obtained from Sigma-Aldrich. (St. Louis, MO, USA) Go Taq^® DNA polymerase (M3005) was purchased from Promega. NI PXIe-1060Q (NI) was obtained from National Instruments. QuickExtract™ DNA extraction solution 1.0 (QE09050) was obtained from Epicentre. The thermal cycler (C1000Touch™) was obtained from Bio-Rad (Hercules, CA, USA). LIAS Slite 140 was purchased from Avegene Life Sciences. The plotting cutter (FC4600C-50 PRO) was obtained from GRAPHTEC (Tokyo, Japan).