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Fmoc l phe oh

Manufactured by GL Biochem
Sourced in China

Fmoc-L-Phe-OH is an amino acid derivative used in chemical synthesis. It serves as a building block in the production of peptides and other organic compounds.

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4 protocols using fmoc l phe oh

1

Peptide Synthesis and Characterization

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2-chlorotrityl chloride resin (100–200 mesh; 1% DVB; 1.01 mmol/g), Fmoc-L-Phe-OH, Fmoc-Gly-OH and Fmoc-L-Ala-OH were purchased from Shanghai GL biochem (China). DMEM, α-MEM medium and Phosphate Buffered Saline (PBS, 0.0067 M) were provided by Thermo Fisher Scientific Corporation (USA). Fetal bovine serum (FBS, Gibco, South America origin) was bought from Life Technologies Corporation (USA). All the other compounds were obtained by Chengdu Best-reagent Corporation (China) and used without further purification. 1H NMR spectra were collected on a Bruker AVII-400 (400 MHz, USA) using DMSO‑d6 as a solvent. Liquid chromatography-mass spectrometry (LC-MS) analysis was performed on a Finnigan TSQ Quantum Ultra LC-MS system with an electrospray ionization (ESI) source.
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2

Gelatin-Based Biomaterial Synthesis

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Gelatin was purchased from Avantor, Inc. Methacrylic anhydride (MA) was purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). N, N-Diisopropylethylamine (DIPEA), 4-biphenyl acetic acid (BPAA), and trifluoroacetic acid (TFA) were purchased from Best-reagent Corporation (Chengdu, China). Fmoc-L-Phe-OH, Fmoc-Gly-Oh, and 2-chlorotrityl chloride resin were provided from Shanghai GL biochem (Shanghai, China). Phosphate buffered saline (PBS) was bought from Invitrogen. Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), type II collagenase, trypsin, and penicillin/streptomycin (PS) were bought from Gibco (USA). α-MEM medium was purchased from Hyclone (USA). Type I collagen was extracted and purified from new-born calf skin in our laboratory. All other chemicals were used as received unless otherwise specified.
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3

Photocatalytic Peptide Synthesis

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5-Mono(4-carboxyphenyl)-10,15,20-triphenyl porphine (MCpP) was purchased from Frontier Scientific (Logan, U.S.A.), Fmoc-L-Phe-OH from GL Biochem (Shanghai, China), O-(1H-benzotriazole-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU) and N-hydroxybenzotriazole anhydrous (HOBt) from Chem-Impex International (Wood Dale, IL, U.S.A.), N,N′-diisopropylethylamine (DIEA), triisopropylsilane (TIS), hexafluoroisopropanol (HFIP), tris(2,2′-bipyridyl)dichlororuthenium(II) hexahydrate (Ru(bpy)3Cl2·6H2O), L-glutamic dehydrogenase from bovine liver (GDH) and α-ketoglutaric acid from Sigma-Aldrich (Rehovot, Israel), nicotinamide adenine dinucleotide (NAD+) from Aladdin (Shanghai, China), triethanolamine (TEOA) from Sinopharm (Shanghai, China), Fmoc-L-Phe-Wang resin from Merck (Darmstadt, Germany), and trifluoroacetic acid (TFA), dichloromethane (DCM), and N,N-dimethylformamide (DMF) of peptide synthesis purity grade from Bio-Lab (Jerusalem, Israel). All materials were used as received without further purification. Water used was processed by a Millipore purification system (Darmstadt, Germany) with a minimum resistivity of 18.2 MΩ cm.
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4

Solid-Phase Peptide Synthesis of Diverse Biomolecules

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All chemicals were purchased from commercial vendors and used without further purification. Fmoc-L-Gly-OH, Fmoc-L-Leu-OH, Fmoc-L-Ile-OH, Fmoc-L-Val-OH, Fmoc-L-Lys(Boc)-OH, Fmoc-L-Lys(Alloc)-OH, Fmoc-L-Ala-OH, Fmoc-L-Cys(Trt)-OH, Fmoc-L-Gln(Trt)-OH, Fmoc-L-Asn(Trt)-OH, Fmoc-L-Glu(OtBu)-OH, Fmoc-L-Asp(OtBu)-OH, Fmoc-L-Arg(Pbf)-OH, Fmoc-L-Phe-OH, Fmoc-L-Trp(Boc)-OH, Fmoc-His(Boc)-OH, Fmoc-L-Ser(tBu)-OH, Fmoc-L-Thr(tBu)-OH, Fmoc-L-Tyr(tBu)-OH, Fmoc-L-Pro-OH, Boc-L-Asp(OtBu)-OH, and Fmoc-L-Met-OH were purchased from GL Biochem. Fmoc-Rink amide resin was purchased from Tjhecheng. Bromoacetic acid, [2-[2-(Fmoc-amino)ethoxy]ethoxy]acetic acid, N,N′-diisopropylcarbodiimide (DIC), ethyl cyanoglyoxylate-2-oxime (Oxyma), N,N-dimethylformamide (DMF), dichloromethane (DCM), diethyl ether, trifluoroacetic acid, triisoproprylsilane, 4-methyl pepiridine and HPLC-grade acetonitrile were obtained from Energy Chemical.
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