Log In Sign up
The largest database of trusted experimental protocols

Legend max mouse il 10 il 12 elisa kit

Manufactured by BioLegend
Visit Supplier
Sourced in United States

The LEGEND MAX™ Mouse IL‐10/IL‐12 ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of mouse interleukin-10 (IL-10) and interleukin-12 (IL-12) in cell culture supernatants, serum, and plasma.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

IL-10 (167 citations) IL-12 (68 citations) ELISA MAX kits (48 citations) Mouse ELISA kits (37 citations) ELISAs (15 citations) Mouse IL-10 (4 citations) Mouse IL-12 (2 citations)

2 protocols using legend max mouse il 10 il 12 elisa kit

1

Quantifying Cytokine Levels in RAW Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The supernatant from RAW cultures, previously treated for 48 h with IL‐4 (20 ng·ml−1) and HA‐PeiPLGA NPs in the absence or presence of IL‐10 receptor antibody (1.2 μg·ml−1), was collected and the levels of murine IL‐10 and IL‐12 measured using a LEGEND MAX™ Mouse IL‐10/IL‐12 ELISA Kit (BioLegend, Cat#430607 and Cat#430707, respectively) according to the manufacturer's instructions. Five independent assays with at least three replicates were performed for each experiment.
Cavalcante R.S., Ishikawa U., Silva E.S., Silva‐Júnior A.A., Araújo A.A., Cruz L.J., Chan A.B, & de Araújo Júnior R.F. (2021). STAT3/NF‐κB signalling disruption in M2 tumour‐associated macrophages is a major target of PLGA nanocarriers/PD‐L1 antibody immunomodulatory therapy in breast cancer. British Journal of Pharmacology, 178(11), 2284-2304.
+ Open protocol
+ Expand
2

Murine IL-10 and IL-12 Expression After NP3 and abIL-10R Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were incubated and treated with 2.5 μg/ml NP3 (RA)-CHO, 1.2 μg/ml abIL-10R, and 2.5 μg/ml NP3 (RA)-CHO+1.2 μg/ml abIL-10R for 48 hours. After 48 hours of incubation, the supernatants were collected and murine IL-10 and IL-12 levels were determined by means of a LEGEND MAX™ Mouse IL-10/IL-12 ELISA Kit (Bio-Legend, San Diego, CA, USA) according to the protocol of the manufacturer. Experiments were performed in triplicate and repeated twice.
Júnior R.F., Lira G.A., Schomann T., Cavalcante R.S., Vilar N.F., de Paula R.C., Gomes R.F., Chung C.K., Jorquera-Cordero C., Vepris O., Chan A.B, & Cruz L.J. (2023). Retinoic acid-loaded PLGA nanocarriers targeting cell cholesterol potentialize the antitumour effect of PD-L1 antibody by preventing epithelial-mesenchymal transition mediated by M2-TAM in colorectal cancer. Translational Oncology, 31, 101647.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!