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Multiplex cytokine kit

Manufactured by Bio-Rad
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Sourced in United States

The Multiplex cytokine kit is a laboratory instrument used for the simultaneous detection and quantification of multiple cytokines in a single sample. The kit utilizes bead-based technology and flow cytometry principles to provide a comprehensive analysis of cytokine profiles.

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Lab products found in correlation

Bio plex 100 system, by Bio-Rad (1 mentions) Bio plex 200 system, by Bio-Rad (1 mentions)

3 protocols using multiplex cytokine kit

1

Multiplex Cytokine Profiling in DHSC

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Cytokine levels were assessed in DHSC (L4–6) homogenates using a commercially available multiplex cytokine kit (Bio-Rad Laboratories) as previously described (Janes et al., 2014 (link)).
Stockstill K., Doyle T.M., Yan X., Chen Z., Janes K., Little J.W., Braden K., Lauro F., Giancotti L.A., Harada C.M., Yadav R., Xiao W.H., Lionberger J.M., Neumann W.L., Bennett G.J., Weng H.R., Spiegel S, & Salvemini D. (2018). Dysregulation of sphingolipid metabolism contributes to bortezomib-induced neuropathic pain. The Journal of Experimental Medicine, 215(5), 1301-1313.
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2

Quantifying IL-1β in Cell Cultures

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Protein levels of IL-1β were measured in culture supernatants using Luminex multianalyte technology on the Bio-Plex 100 system and multiplex cytokine kit (Bio-Rad, Hercules, CA, USA), the sensitivity of which was < 1 pg/mL.
Ascone G., Cao Y., Jansen I.D., Di Ceglie I., van den Bosch M.H., Blom A.B., van Lent P.L., Everts V, & de Vries T.J. (2020). Increase in the Number of Bone Marrow Osteoclast Precursors at Different Skeletal Sites, Particularly in Long Bone and Jaw Marrow in Mice Lacking IL-1RA. International Journal of Molecular Sciences, 21(11), 3774.
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3

Quantification of IL-6 in Chondrocytes

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Luminex multianalyte technology on the Bio-Plex 200 system (Bio-Rad) in combination with multiplex cytokine kit (Bio-Rad) was used to study levels of IL-6 in 50 ml of chondrocyte culture supernatants after rhTGF-b1 stimulation. Samples below detection limit (0.40 pg/ml) were set at lowest measurable concentration to perform statistical analysis.
Wiegertjes R., van Caam A., van Beuningen H., Koenders M., van Lent P., van der Kraan P., van de Loo F, & Blaney Davidson E. (2019). TGF-β dampens IL-6 signaling in articular chondrocytes by decreasing IL-6 receptor expression. Osteoarthritis and cartilage, 27(8).
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