ITE was a gift from Dr Jiasheng Song, (AhR Pharmaceuticals, Inc., Madison, WI, USA). Anti-CYP450 isoenzyme 1A1 antibody (ab126828), anti-CYP450 isoenzyme 1B1 antibody (ab33586), anti-CYP450 isoenzyme 1A2 antibody (ab56073), CYP3A4 antibody (ab135813), CYP3A5 antibody (ab108624), CYP2D6 antibody (ab62204), CYP2C9 antibody (ab150364) and CYP2E1 antibody (ab90564) were all purchased from Abcam (Cambridge, MA, USA).
Ab33586
Manufactured by Abcam
Sourced in United States
Ab33586 is a laboratory equipment product. It is designed for use in various scientific and research applications. The core function of this product is to perform specific tasks related to the research or analysis being conducted. No further details about the intended use or capabilities of this product are available.
Automatically generated - may contain errors
Lab products found in correlation
Ab3568, by Abcam (2 mentions)
Ab108624, by Abcam (1 mentions)
Ab135813, by Abcam (1 mentions)
Hrp conjugated anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)
Nitrocellulose membrane, by Pall Corporation (1 mentions)
β actin, by Proteintech (1 mentions)
Ab194697, by Abcam (1 mentions)
αmem glutamax, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Harvard Bioscience (1 mentions)
Fetal calf serum (fcs), by Leibniz Institute DSMZ (1 mentions)
Ab2770, by Abcam (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
3 protocols using ab33586
1
Cytochrome P450 Antibody Validation
2
Liver Protein Extraction and Western Blot
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Total cellular protein of liver tissues was extracted by RIPA lysis buffer (150 M of NaCl, 1% Triton X-100, 0.5% deoxycholate, 0.1% SDS, and 50 mM Tris (pH 7.4)) containing protease inhibitors. 20 μg soluble proteins were separated by 8 ~ 12% SDS-PAGE and were transferred onto a nitrocellulose membrane (Pall Corporation, NY, USA). After being blocked with 5% fat free milk, the membranes were incubated with primary antibodies against caspase3 (#9662), cleaved caspase 3 (#9664), CYP1A2 (#14719) (Cell Signaling Technology, MA, USA), CYP1A1 (#ab3568), CYP1B1 (#ab33586), GSTT1 (#ab199337), GSTM1 (#ab113432), UGT1A1 (#ab194697) (Abcam, Cambridge, UK), and β-actin (#60008; Proteintech Group, AL, USA). Immunolabeling was visualized with HRP-conjugated anti-rabbit IgG or anti-mouse IgG (Santa Cruz Biotechnology, CA, USA). The density of the specific bands was quantified using ImageJ software.
3
Cytospin Immunostaining of Myeloid Cells
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Cytospin preparations of moDCs, LCs, MUTZ-3, THP-1 and U937 (~ 0.1 x 10 6 cells) were stained with monoclonal antibodies against CYP1A1 (ab3568), CYP1B1 (ab33586) and AhR (ab2770) (Abcam, Cambridge, UK) each for 1 h and protein expression was detected using a labeled streptavidin-biotin in Minimum Essential Medium alpha (MEM-α) + GlutaMAX™ (Gibco/Invitrogen, Darmstadt, Germany) supplemented with ribonucleosides and deoxyribonucleosides, 20% FCS (Biochrom, Berlin, Germany), 2 mM L-Glutamine (Life Technologies, Darmstadt, Germany), 50 µM β-mercaptoethanol (Sigma-Aldrich, Munich, Germany) and 10% of conditioned medium from the renal cell carcinoma cell line 5637 at 37°C in a 5% CO2 humidified incubator. Cells were subcultured at a split ratio of 1:2 twice per week. Cells were stimulated with benzo[a]anthracene on days 6 -7.
5637, a renal cell carcinoma cell line, was purchased from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany, ACC35) and was cultured in RPMI medium supplemented with 10% FCS. The cells were subcultured at a split ratio of 1:4 to 1:5 and passaged every 3-4 days.
5637, a renal cell carcinoma cell line, was purchased from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany, ACC35) and was cultured in RPMI medium supplemented with 10% FCS. The cells were subcultured at a split ratio of 1:4 to 1:5 and passaged every 3-4 days.
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