Human umbilical vein endothelial cells (huvec)

Human hepatocellular carcinoma cells MHCC-97H and HCC-LM3, and human umbilical vein endothelial cells (HUVEC) were purchased from Cell Bank, Chinese Academy of Sciences (Shanghai, China). HCC-LM3 cells were cultured in DMEM, MHCC-97H and HUVEC cells were cultured in RPMI-1640, supplemented with 10% Fetal Bovine Serum (FBS, Wisent, Inc., CAN, Quebec, QC, Canada) and 100 U/ml of streptomycin and penicillin (GIBCO, Thermo Fisher Scientific Inc., Chino, CA, USA) at 37 °C with 5% CO₂.
The anchorage-independent growth of cells was performed in six-well plate coated with poly-2-hydroxyethylmethacrylate (poly-HEMA, Sigma-Aldrich, St. Louis, MO, USA). poly-HEMA powder was dissolved to 12 mg/mL in 95% ethanol.

Human colorectal cancer (COLO 205, CCL-222), breast cancer (MCF-7, HTB-22), chronic myelogenous leukemia (K-562, CCL-243), and umbilical vein/vascular endothelium (HUVEC, CRL-1730) cell lines were obtained from the American Type Culture Collection. The COLO 205, MCF-7, K-562, and HUVEC cell lines were maintained in Roswell Park Memorial Institute (RPMI 164, Wisent, Montreal, QC, CANADA), Eagle′s Minimum Essential Medium (EMEM, Wisent, Montreal, QC, CANADA), Iscove’s Modified Dulbecco’s Medium (IMDM, Wisent, Montreal, QC, CANADA), and Dulbecco′s Modified Eagle Medium (DMEM, Wisent, Montreal, QC, CANADA) supplemented with 10% fetal bovine serum (Capricorn, Ebsdorfergrund, Germany), 100 U/mL penicillin, and 100 µg/mL streptomycin (Wisent, Montreal, QC, CANADA), respectively. All of the cell lines were cultured at 37 °C in a humidified atmosphere containing 5% CO₂.