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Anti core antibody clone c7 50

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The Anti-core antibody (clone C7-50) is a laboratory reagent used for the detection and identification of the core antigen of various organisms. This antibody is produced in mouse and can be used in various immunoassay techniques, such as ELISA and Western blotting, to detect the presence of the target antigen. The core function of this antibody is to serve as a tool for researchers to investigate the presence and characteristics of the core antigen.

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Anti-core C7-50 (3 citations) C7-50) (2 citations)

2 protocols using anti core antibody clone c7 50

1

HCV Infectivity Titer Measurement

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The HCV infectivity titers were measured by indirect immunostaining as described previously21 (link),46 (link). For the indirect immunofluorescence analysis, the cells were fixed with cold methanol and incubated with an anti-core antibody (clone C7–50; Abcam, Cambridge, UK) and subsequently with fluorescent labeled secondary antibody. The infectivity titer was calculated the focus-forming units per mL and expressed as relative to JFH1/5AC-wt.
Nitta S., Asahina Y., Kato T., Tsuchiya J., Inoue-Shinomiya E., Sato A., Tsunoda T., Miyoshi M., Kawai-Kitahata F., Murakawa M., Itsui Y., Nakagawa M., Azuma S., Kakinuma S., Hikita H., Takehara T, & Watanabe M. (2019). Impact of novel NS5A resistance-associated substitutions of hepatitis C virus detected in treatment-experienced patients. Scientific Reports, 9, 5722.
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2

Measuring HCV Infectivity Titers

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The HCV infectivity titers were measured by indirect immunostaining as described previously45 (link). Briefly, Huh-7.5.1 cells seeded into 96-well plates were cultured overnight and infected with serially diluted inocula. The inocula were collected from the culture media or cells from HCV-RNA transfected Huh7-25 cells. Three days after infection, indirect immunostaining was performed with an anti-core antibody (clone C7-50; Abcam, Cambridge, UK). The infectivity titer was expressed as the focus-forming units (FFU) per mL.
Nitta S., Asahina Y., Matsuda M., Yamada N., Sugiyama R., Masaki T., Suzuki R., Kato N., Watanabe M., Wakita T, & Kato T. (2016). Effects of Resistance-Associated NS5A Mutations in Hepatitis C Virus on Viral Production and Susceptibility to Antiviral Reagents. Scientific Reports, 6, 34652.
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