Hela and A549 cells were grown in DMEM medium (PAN Biotech, Aidenbach, Germany) supplemented with 10% fetal bovine serum (FBS, GE Healthcare) and 100 µg/ml penicillin/streptomycin (Pen/Strep, PAN Biotech). 116 cells were grown in MEM eagle medium (PAN Biotech) supplemented with 10% FBS, 100 µg/ml penicillin/streptomycin and 100 mg/ml hygromycin B (PAN Biotech). Primary human skeletal myoblasts (HSKM) were grown in DMEM supplemented with 20% FBS, 1% gentamycin and 1x Glutamax (Thermo Fischer Scientific). All cells were cultured at 37 °C and 5% CO2 in a standard tissue culture incubator.
Penicillin streptomycin pen strep
Manufactured by PAN Biotech
Sourced in Germany
Penicillin/Streptomycin (Pen/Strep) is a commonly used antibiotic solution for cell culture applications. It contains a mixture of penicillin and streptomycin, two broad-spectrum antibiotics that work to prevent bacterial contamination in cell cultures.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by PAN Biotech (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Gentamycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by GE Healthcare (1 mentions)
Mem eagle medium, by PAN Biotech (1 mentions)
Penicillin streptomycin, by PAN Biotech (1 mentions)
Dmem medium, by PAN Biotech (1 mentions)
Hygromycin b, by PAN Biotech (1 mentions)
Ascorbic acid, by Carl Roth (1 mentions)
Propidium iodide, by Santa Cruz Biotechnology (1 mentions)
Annexin 5 apoptosis detection kit, by Santa Cruz Biotechnology (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Serva Electrophoresis (1 mentions)
2 7 dichlorodihydrofluorescein diacetate h2dcf da, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by PAN Biotech (1 mentions)
Bortezomib, by Adooq (1 mentions)
Rpmi 1640, by PAN Biotech (1 mentions)
3 protocols using penicillin streptomycin pen strep
1
Cell Culture Conditions for Multiple Cell Types
2
Evaluation of Cytotoxic Compounds in Cancer Cells
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Roswell Park Memorial Institute (RPMI) media 1640, fetal bovine serum (FBS), penicillin/streptomycin (pen/strep) [10,000 U/ml; 10 mg/ml] and trypsin-EDTA (0.05% Trypsin, 0.02% EDTA in PBS) were purchased from PAN Biotech (Aidenbach, Germany). CDDP, RV and 2´-7´-dichlorodihydrofluorescein-diacetate (H2DCF-DA) were from Sigma-Aldrich (Steinheim, Germany). CDDP was dissolved and subsequently diluted in 0.9% saline. RV and H2DCF-DA were dissolved in DMSO and subsequently diluted with 1x PBS (10x PBS: 1.4 M NaCl, 30 mM KCl, 80 mM Na2HPO4 x 2 H2O, 15 mM KH2PO4). EA was purchased from Fluka Chemie AG (Buchs, Switzerland). The compound was dissolved in DMSO and subsequently diluted in PBS. Ascorbic acid was from Carl Roth (Karlsruhe, Germany) and was dissolved in 0.9% saline. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Serva (Heidelberg, Germany) and was dissolved in PBS at a concentration of 5 mg/ml. Propidium iodide and the annexin V apoptosis detection kit were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Propidium iodide was dissolved in PBS.
3
Bortezomib and TMP269 Treatment Protocol
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The culture medium RPMI 1640 and DMEM, Penicillin/streptomycin (pen/strep) (10,000 U/mL; 10 mg/mL) and trypsin-EDTA (0.05% Trypsin, 0.02% EDTA in Phosphate Buffer Saline) were related from PAN-Biotech (Aidenbach, Germany). Bortezomib and TMP269 were ordered from AdooQ (AdooQ Bioscience, Irvine, California, USA). Both compounds were dissolved in DMSO at 10 mM. Further dilutions were made with the appropriate culture medium. A maximum concentration of 1% DMSO was used in all experiments.
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