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Alexa680 coupled secondary antibodies

Manufactured by Thermo Fisher Scientific
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Sourced in Germany

Alexa680-coupled secondary antibodies are fluorescent-labeled antibodies designed for use in immunoassays and other applications. The Alexa680 fluorescent dye is coupled to the secondary antibody, allowing for detection and visualization of target proteins or other molecules.

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Lab products found in correlation

Pan akt, by Cell Signaling Technology (2 mentions) Pakt t308, by Cell Signaling Technology (2 mentions) Ps6 s235 236, by Cell Signaling Technology (2 mentions) Hsp90 alpha beta, by Santa Cruz Biotechnology (2 mentions) Pakt s473, by Cell Signaling Technology (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions) P27kip1 sc 528, by Santa Cruz Biotechnology (1 mentions) P27kip1, by Santa Cruz Biotechnology (1 mentions)

2 protocols using alexa680 coupled secondary antibodies

1

Western Blot Analysis of AKT and S6 Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell lysates were prepared and isolated proteins separated in SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes and incubated at 4°C overnight with the following primary antibodies: pAKT-T308 (#2965, RRID:AB_2255933), pAKT-S473 (#4060, RRID:AB_2315049), pan-AKT (#4691, RRID:AB_915783), p-S6-S235/236 (#4858, RRID:AB_916156; all from Cell Signaling Technology) and p27Kip1 (sc528, RRID:AB_632129), Skp2 (sc7164, RRID:AB_2187650) and Hsp90 alpha/beta (sc13119, RRID:AB_675659; all from Santa Cruz Biotechnology). Primary antibodies were followed by Alexa680-coupled secondary antibodies (Thermo Fisher Scientific) and detected by the Odyssey Infrared Imaging System (Licor, Bad Homburg, Germany).
Falcomatà C., Bärthel S., Ulrich A., Diersch S., Veltkamp C., Rad L., Boniolo F., Solar M., Steiger K., Seidler B., Zukowska M., Madej J., Wang M., Öllinger R., Maresch R., Barenboim M., Eser S., Tschurtschenthaler M., Mehrabi A., Roessler S., Goeppert B., Kind A., Schnieke A., Robles M.S., Bradley A., Schmid R.M., Schmidt-Supprian M., Reichert M., Weichert W., Sansom O.J., Morton J.P., Rad R., Schneider G, & Saur D. (2021). Genetic Screens Identify a Context-Specific PI3K/p27Kip1 Node Driving Extrahepatic Biliary Cancer. Cancer Discovery, 11(12), 3158-3177.
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2

Western Blot Analysis of AKT Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell lysates were prepared, and isolated proteins were separated in SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes, and incubated at 4°C overnight with the following primary antibodies: pAKT-T308 (#2965, RRID:AB_2255933), pAKT-S473 (#4060, RRID:AB_2315049), pan-AKT (#4691, RRID:AB_915783), p-S6-S235/236 (#4858, RRID:AB_916156; all from Cell Signaling Technology) and p27Kip1 (sc528, RRID:AB_632129), Skp2 (sc7164, RRID:AB_2187650), and Hsp90 alpha/beta (sc13119, RRID:AB_675659; all from Santa Cruz Biotechnology). Primary antibodies were followed by Alexa680-coupled secondary antibodies (Thermo Fisher Scientific) and detected by the Odyssey Infrared Imaging System (Licor).
Falcomatà C., Bärthel S., Ulrich A., Diersch S., Veltkamp C., Rad L., Boniolo F., Solar M., Steiger K., Seidler B., Zukowska M., Madej J., Wang M., Öllinger R., Maresch R., Barenboim M., Eser S., Tschurtschenthaler M., Mehrabi A., Roessler S., Goeppert B., Kind A., Schnieke A., Robles M.S., Bradley A., Schmid R.M., Schmidt-Supprian M., Reichert M., Weichert W., Sansom O.J., Morton J.P., Rad R., Schneider G, & Saur D. (2021). Genetic Screens Identify a Context-Specific PI3K/p27Kip1 Node Driving Extrahepatic Biliary Cancer. Cancer Discovery, 11(12), 3158-3177.
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