A volume of 200 mL of inulin 30% aqueous solution was added to 400 mL of strawberry aqueous extract. The solution was dehydrated by Laboratory Scale Spray Dryer (Lab-Plant SD-05, Keison International Ltd, UK) according to Beirão-da-Costa et al. (2013) (link) and freeze dried (LyoQuest, Azbil Telstar Technologies). The microencapsulates were stored in petri dishes in desiccators in the dark until analysis. To measure the total anthocyanin content and total phenolic content, and to evaluate the antioxidant capacity, the SME were prepared by weighing 1.5 g per replicate into 20 mL falcon tubes, then adding 10 mL of water and keeping them hydrated for 24 hours. The solution was stirred (Ultraturrax T 25) for 5 min and centrifuged (Sigma and Laborzentrifugen, 2k15) at 5000 rpm for 10 minutes. Finally, the supernatant was collected and filtered through a Whatman no 41 filter (Whatman, Maidstone, UK) and brought to a known volume with deionized water. The extracts were stored at -18 о C until analysis.
Whatman no 41 filter
Manufactured by Cytiva
Sourced in United Kingdom
Whatman no 41 filter is a high-quality cellulose filter paper designed for general laboratory filtration applications. It features a medium-fast flow rate and retains a wide range of particle sizes. The filter paper is made from high-purity cellulose and undergoes rigorous quality control to ensure consistent performance.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using whatman no 41 filter
1
Microencapsulation of Strawberry Extract
2
Anthocyanin Extraction from Fruit
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Approximately 500 g of fruit per replicate was homogenized from a batch of 5 kg of fruit and used in the preparation of the SAE. Anthocyanins were extracted from the fruit using deionized water at pH = 4.6 (250 g•L -1 ) by grinding in an Ultra-Turrax (IKA T-25, Janke and Kunkel) for 3 minutes at room temperature (20°C). The extracts were placed in a magnetic stirrer (Are, VelpScientifica) and stirred for 15 minutes at room temperature (20°C), and centrifuged (Sigma and Laborzentrifugen, 1k15) at 3000 rpm for 10 min at 5°C. Finally, the supernatant was collected and filtered through a Whatman no 41 filter (Whatman, Maidstone, UK) and brought to a known volume with deionized water. The extracts were stored at -18 о C until analysis.
3
Extraction and Quantification of Cocoa Bean Free Amino Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the extraction of free amino acids, 4.5 g of finely grinded dry cocoa beans were dispersed under magnetic stirring in 40 mL of sodium citrate buffer (0.2 mol/L, pH 2.2) for 40 min. The dispersion was sonicated for 5 min and filtered using a Whatman No. 41 filter (Whatman plc, Maidstone, UK). An aliquot of 10 mL of the filtrate was deproteinated by adding 10 mL solution of 75 g/L sulfosalicylic acid at pH 1.75, under magnetic stirring, for 5 min. After the addition of 250 µL of Nor-Leucine as internal standard the solution was brought to the final volume of 25 mL and filtered using a Whatman No. 42 filter (Whatman) and subsequently using a 0.2 µm pore size syringe filter. A 100 µL volume of the filtered sample was used for quantification of free amino acids by ion exchange chromatography (IEC) using a Biochrom 30+ chromatograph equipped with an automatic sampler, as described by Hogenboom et al. [13 (link)]. An unfermented dry cocoa sample was also analysed to evaluate the changes in the concentration of free amino acids due to the fermentation process.
4
Microencapsulation of Strawberry Extract
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A volume of 200 mL of inulin 30% aqueous solution was added to 400 mL of strawberry aqueous extract. The solution was dehydrated by Laboratory Scale Spray Dryer (Lab-Plant SD-05, Keison International Ltd, UK) according to Beirão-da-Costa et al. (2013) (link) and freeze dried (LyoQuest, Azbil Telstar Technologies). The microencapsulates were stored in petri dishes in desiccators in the dark until analysis. To measure the total anthocyanin content and total phenolic content, and to evaluate the antioxidant capacity, the SME were prepared by weighing 1.5 g per replicate into 20 mL falcon tubes, then adding 10 mL of water and keeping them hydrated for 24 hours. The solution was stirred (Ultraturrax T 25) for 5 min and centrifuged (Sigma and Laborzentrifugen, 2k15) at 5000 rpm for 10 minutes. Finally, the supernatant was collected and filtered through a Whatman no 41 filter (Whatman, Maidstone, UK) and brought to a known volume with deionized water. The extracts were stored at -18 о C until analysis.
5
Anthocyanin Extraction from Fruit
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Approximately 500 g of fruit per replicate was homogenized from a batch of 5 kg of fruit and used in the preparation of the SAE. Anthocyanins were extracted from the fruit using deionized water at pH = 4.6 (250 g•L -1 ) by grinding in an Ultra-Turrax (IKA T-25, Janke and Kunkel) for 3 minutes at room temperature (20°C). The extracts were placed in a magnetic stirrer (Are, VelpScientifica) and stirred for 15 minutes at room temperature (20°C), and centrifuged (Sigma and Laborzentrifugen, 1k15) at 3000 rpm for 10 min at 5°C. Finally, the supernatant was collected and filtered through a Whatman no 41 filter (Whatman, Maidstone, UK) and brought to a known volume with deionized water. The extracts were stored at -18 о C until analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!