Triclosan tcs

Manufactured by Merck Group

Sourced in United States

Triclosan (TCS) is a synthetic antimicrobial agent used in various lab equipment and products. It has a chemical structure that allows it to inhibit the growth of bacteria, fungi, and some viruses. TCS is commonly used as a preservative and disinfectant in laboratory settings.

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Triclosan

4 protocols using triclosan tcs

Autophagy Monitoring Using Fluorescent LC3 Constructs

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The antibodies used in the experiments were purchased from Cell Signaling Technology (Maryland, USA), the triclosan (TCS) came from Sigma (Missouri, USA), and the other reagents, unless stated otherwise, were all purchased from Sigma (Missouri, USA). The pmRFP-LC3 is mammalian expression of rat LC3 fused to mRFP, the ptfLC3 is mammalian expression of rat LC3 fused to mRFP and EGFP, and the pEGFP-LC3 is mammalian expression of rat LC3 fused to EGFP (Kimura et al., 2007 (link)). Lactobacillus, Escherichia coli (E. coli), and Enterococcus faecalis (E. faecalis) were isolated from normal mouse feces. S. typhimurium ATCC 14028 and C. albicans SC5314 were kept by our laboratory.

Wang C., Yu Z., Shi X., Tang X., Wang Y., Wang X., An Y., Li S., Li Y., Wang X., Luan W., Chen Z., Liu M, & Yu L. (2018). Triclosan Enhances the Clearing of Pathogenic Intracellular Salmonella or Candida albicans but Disturbs the Intestinal Microbiota through mTOR-Independent Autophagy. Frontiers in Cellular and Infection Microbiology, 8, 49.

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Methacrylated Collagen Hydrogel Synthesis

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Collagen was purchased from MSBIO, Inc. (Seongnam, Kyunggi, Korea). Methacrylic anhydride (MA), sodium phosphate monobasic, sodium phosphate dibasic, 2-hydroxypropyl-beta-cyclodextrin (HP-β-CD), and triclosan (TCS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Riboflavin 5′-monophosphate sodium salt (riboflavin) as a photoinitiator was obtained from Santa Cruz (Santa Cruz, CA, USA). Dialysis membrane tubes (cut-off: 25 kDa and 3.5–5.0 kDa; Spectrum Laboratories Inc., Rancho Dominguez, CA, USA) were used for methacrylated collagen (ColMA). The NIH3T3 mouse fibroblast cell line was obtained from the Korean Cell Line Bank (Seoul, Korea).

Jin L., Park K., Yoon Y., Kim H.S., Kim H.J., Choi J.W., Lee D.Y., Chun H.J, & Yang D.H. (2021). Visible Light-Cured Antibacterial Collagen Hydrogel Containing Water-Solubilized Triclosan for Improved Wound Healing. Materials, 14(9), 2270.

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Preparation and Characterization of Triclosan

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Triclosan (TCS; 99% purity; Sigma-Aldrich) was prepared in BT (Tyrodes buffer containing bovine serum albumin [BSA]) without use of organic solvent (Weatherly et al., 2013 (link)) and diluted to deliver non-cytotoxic concentrations (Palmer et al., 2012 (link)) checked by UV-Vis spectrophotometry (Weatherly et al., 2013 (link)). Gramicidin (Sigma- Aldrich) was dissolved in 100% DMSO (Sigma-Aldrich) and diluted with BT.

Sangroula S., Baez Vasquez A.Y., Raut P., Obeng B., Shim J.K., Bagley G.D., West B.E., Burnell J.E., Kinney M.S., Potts C.M., Weller S.R., Kelley J.B., Hess S.T, & Gosse J.A. (2020). Triclosan Disrupts Immune Cell Function by Depressing Ca2+ Influx Following Acidification of the Cytoplasm. Toxicology and applied pharmacology, 405, 115205.

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Quantification of Triclosan and Metabolites

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Analytical standards (99.9% purity) for triclosan (TCS) and methyltriclosan (MTCS) were purchased from Sigma-Aldrich, Shanghai, China. The chemical structure and molecular weight of TCS and MTCS are shown in Supplementary Fig. 1. Five medium-chain fatty acids (pentanoic acid, hexanoic acid, heptanoic acid, octanoic acid and nonanoic acid) were purchased from J&K Chemical, Shanghai, China. HPLC-grade m ethanol, ethanol, ethylene glycol, acetone, acetonitrile, and dimethyl sulfoxide were purchased from Merck, Shanghai, China.
Stock standard solutions (1000 μg mL -1 ) for TCS and MTCS were prepared by dissolving each chemical in methanol and stored at 4 °C until use. Working solutions were diluted with Milli-Q 18 MΩ ultrapure water (Millipore, Bedford, MA, USA) and methanol (v/v = 50:50) to prepare secondary mixed stock solutions. All working solutions were prepared fresh weekly and stored at 4 °C.

Gao M., Wang J., Zhang X., Dahlgren R.A., Ru S, & Wang X. (2018). Integrated disperser freezing purification with extraction using fatty acid-based solidification of floating organic-droplet (IDFP-EFA-SFO) for triclosan and methyltriclosan determination in seawater, sediment and seafood. Marine pollution bulletin, 137.

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