The following mAbs were obtained from eBioscience (San Diego, CA, USA) or BioLegend (San Diego, CA, USA) for FACS analysis and other experiments: FITC-labeled anti-CD4 (RMA4-5), CD45 (30-F11), CD80 (16-10-A1), CD86 (GL1), MHC II (M5/114.15.2), CD44 (IM7), CD69 (H1.2F3), CD62L (MEL-14) and CD11b (M1/70); PE-labeled anti-CD8 (53–6.7), F4/80 (BM8), and IFN-γ (XMG1.2), TLR2 (6C2), CD154 (MR1), CD25 (PC61.5); PerCP/Cy5.5-labeled anti-mouse CD11b (M1/70), Ly-6C antibody (HK1.4), and IFN-γ (XMG1.2); PE/Cy7-labeled anti-IL-2 (JES6-5H4); APC-labeled anti-Ly6-G (1A8), IL-12/23p40 (C17.8), iNOS (CXNFT), TNF-α (MP6-XT22), CD206 (MR6F3), and CXCR3 (CXCR3-173). PE-labeled anti-mouse Tmem119 (106–6) was obtained from Abcam (Cambridge, MA, USA). The JEV epitope peptide of CD4+ T cells (NS1132-145 [TFVVDGPETKECPD]; NS3563-574 [WCFDGPRTNAIL]) or CD8+ T cells (NS4B215-223 [SAVWNSTTA]) was chemically synthesized at Peptron (Daejeon, Korea). Phorbol-12-Myristate-13-Acetate (PMA), ionomycin, and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Pe labeled anti mouse tmem119 106 6
Manufactured by Abcam
Sourced in United States
PE-labeled anti-mouse Tmem119 (106–6) is a fluorescently-conjugated antibody that binds to the mouse Tmem119 protein. Tmem119 is a transmembrane protein expressed in microglia, the resident immune cells of the central nervous system. The PE label allows for detection and analysis of Tmem119-expressing cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Ns4b215 223, by Peptron (2 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate pma ionomycin, by Merck Group (1 mentions)
Cd11b, by BioLegend (1 mentions)
Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Mp6 xt22, by BioLegend (1 mentions)
2 protocols using pe labeled anti mouse tmem119 106 6
1
Multiparametric FACS Analysis of Immune Cells
2
Antibodies and Peptides for JEV Immunology
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The following mAbs were obtained from eBioscience (San Diego, CA, USA) or BioLegend (San Diego, CA, USA) for FACS analysis and other experiments: FITC-labeled anti-CD4 (RMA4-5), CD45 (30-F11), CD11b (M1/70), CD3 (145-2C11), and CX3CR1 (SA011F11); PE-labeled anti-granzyme B (16G6), CD40L (MR1), CD8 (53–6.7), F4/80 (BM8), IFN-γ (XMG1.2), and CD11c (M1/70); PerCP/Cy5.5-labeled anti-mouse Ly-6C antibody (HK1.4) and IFN-γ (XMG1.2); PE-Cy7-lableled anti-NK1.1 (PK136); APC-labeled anti-Ly6-G (1A8), TNF-α (MP6-XT22), and CD49b-integrin alpha 2 (DX5); and biotin-labeled anti-IL-6 (MP5-32C11) and TNF-α (MP6-XT22). PE-labeled anti-mouse Tmem119 (106-6) was obtained from Abcam (Cambridge, MA, USA). The mAbs against non-structural protein 1 (NS1) and envelope glycoprotein protein (E) of JEV were also obtained from Abcam. The JEV epitope peptide of CD4+ T cells [NS3563−574 (WCFDGPRTNAIL)] or CD8+ T cells [NS4B215−223 (9SAVWNSTTA)] was chemically synthesized at Peptron (Daejeon, Korea). Phorbol-12-Myristate-13-Acetate (PMA) and ionomycin were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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