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Elisa quantitation kits for mouse il 6

Manufactured by R&D Systems
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The Elisa quantitation kits for mouse IL-6 by R&D Systems are designed to quantitatively measure mouse interleukin-6 (IL-6) levels in cell culture supernatants, serum, and plasma.

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ELISA kit for IL-6 ELISA kits for IL-6 IL-6 kits Mouse ELISA kits Mouse IL-6 ELISAs

2 protocols using elisa quantitation kits for mouse il 6

1

Serum Biomarker Quantification in Mice

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Blood samples were collected from six mice, from the buccal artery, which branches off the maxillary artery, allowed to clot at 25°C for 2 h, and centrifuged (2,000 x g, 4°C for 20 min). The supernatant (serum) was then collected. Serum IL‐6 was measured using elisa quantitation kits for mouse IL‐6 (Cat No. M6000B, R&D Systems). HMGB1 was measured using elisa quantitation kits for mouse HMGB‐1 by SRL (Hachioji, Tokyo, Japan).
Suzuki K., Okada H., Takemura G., Takada C., Tomita H., Yano H., Muraki I., Zaikokuji R., Kuroda A., Fukuda H., Nishio A., Takashima S., Suzuki A., Miyazaki N., Fukuta T., Yamada N., Watanabe T., Doi T., Yoshida T., Kumada K., Ushikoshi H., Yoshida S, & Ogura S. (2020). Recombinant thrombomodulin protects against LPS‐induced acute respiratory distress syndrome via preservation of pulmonary endothelial glycocalyx. British Journal of Pharmacology, 177(17), 4021-4033.
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2

Serum Biomarker Quantification in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood samples were collected from the maxillary artery, allowed to clot at room temperature for 2 h, and centrifuged at 2,000×g at 4 °C for 20 min. Blood samples were obtained on six independent samples from individual mice from each group. The supernatant was used to measure the serum IL-6, troponin-I, and syndecan-1 levels using ELISA Quantitation Kits for mouse IL-6 (cat no. M6000B; R&D Systems, Inc.), cardiac troponin-I (CTNI-1-HS; Life Diagnostics, Inc., West Chester, PA, USA), and syndecan-1 (Cat. No. 860.090.192; Diaclone, Besancon Cedex, France).
Kakino Y., Doi T., Okada H., Suzuki K., Takada C., Tomita H., Asano H., Kano S., Wakayama Y., Okuda T., Fukuda H., Nishio A., Kawasaki Y., Kuroda A., Shimada T., Takashima S., Suzuki K., Yoshimura G., Kamidani R., Yasuda R., Fukuta T., Kitagawa Y., Okamoto H., Miyake T., Suzuki A., Yoshida T., Tetsuka N., Yoshida S, & Ogura S. (2022). Recombinant thrombomodulin may protect cardiac capillary endothelial glycocalyx through promoting Glypican-1 expression under experimental endotoxemia. Heliyon, 8(11), e11262.
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