S. rugosoannulata was collected from a field in Yucheng County, Henan Province, China. Trypsin and RPMI1640 were purchased from Gibco (Grand Island, NY, United States). The Cell counting kit-8 (CCK-8) was purchased from Han Heng Biotechnology Co., Ltd. (Shanghai, China). Lipopolysaccharide (LPS) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). The remaining chemicals or reagents were all of laboratory or analytical quality.
Cell counting kit 8 cck 8
Manufactured by Hanheng Biotechnology
Sourced in China
The Cell Counting Kit-8 (CCK-8) is a colorimetric assay used to determine the number of viable cells in a sample. It utilizes a water-soluble tetrazolium salt that is reduced by cellular dehydrogenases, producing a colored formazan dye which can be measured spectrophotometrically. The amount of the formazan dye generated is directly proportional to the number of living cells.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Sodium hydride nah, by Merck Group (1 mentions)
Ascorbic acid vc, by Sinopharm (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
2 protocols using cell counting kit 8 cck 8
1
Isolation and Characterization of S. rugosoannulata
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S. rugosoannulata was collected from a field in Yucheng County, Henan Province, China. Trypsin and RPMI1640 were purchased from Gibco (Grand Island, NY, United States). The Cell counting kit-8 (CCK-8) was purchased from Han Heng Biotechnology Co., Ltd. (Shanghai, China). Lipopolysaccharide (LPS) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). The remaining chemicals or reagents were all of laboratory or analytical quality.
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S. rugosoannulata was collected from a field in Yucheng County, Henan Province, China. Trypsin and RPMI1640 were purchased from Gibco (Grand Island, NY, United States). The Cell counting kit-8 (CCK-8) was purchased from Han Heng Biotechnology Co., Ltd. (Shanghai, China). Lipopolysaccharide (LPS) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). The remaining chemicals or reagents were all of laboratory or analytical quality.
2
Bioactive Compounds from D. huoshanense
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Wild plants of D. huoshanense growing in Huoshan County, Anhui Province, China, were collected. Dimethyl sulfoxide (DMSO), acetonitrile, sodium hydride (NaH), and iodomethane (CH3I) lipopolysaccharide (LPS) were purchased from Sigma Chemical Co., USA. DEAE-cellulose and Sephadex G-100 were purchased from Solarbo Co., Ltd. (Beijing, China). The Cell counting kit-8 (CCk-8) was purchased from Han Heng Biotechnology Co., Ltd. (Shanghai, China). The ascorbic acid (VC) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the other reagents or chemicals were of analytical grade.
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