1,2-dioleoyl-sn-glycerol-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC), 1,2-disteroyl-sn-glycerol-3-phosphocholine (DSPC) cholesterol, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000 (DSPE-PEG2000) and DSPE-PEG3400-Maleimide were purchased from Avanti polar lipids, Miami, FL. 1,1′-dioctadecyl-3,3,3′3′-tetramethylindotricarbocyanine iodide (DiR) was purchased from Invitrogen, Carlsbad, CA. Peptides were synthesized by the Tufts University peptide synthesis core facility using standard FMOC chemistry and Rink-Amide resin (Tufts University, Boston, MA).
1 2 dioleoyl sn glycerol 3 phosphocholine
Manufactured by Avanti Polar Lipids
1,2-dioleoyl-sn-glycerol-3-phosphocholine is a phospholipid compound. It is a synthetic version of the naturally occurring phosphatidylcholine molecule, which is a major component of cell membranes.
Automatically generated - may contain errors
Lab products found in correlation
1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 2000 dspe peg2000, by Avanti Polar Lipids (2 mentions)
Dspe peg3400 maleimide, by Avanti Polar Lipids (2 mentions)
1 2 dipalmitoyl sn glycerol 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
Lab tek chambered coverglass, by Thermo Fisher Scientific (1 mentions)
Streptavidin, by Southern Biotech (1 mentions)
Biotinylated goat f ab 2 anti human λ κ, by Southern Biotech (1 mentions)
Biotinylated annexin 5, by BioLegend (1 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine n cap biotinyl dope cap biotin, by Avanti Polar Lipids (1 mentions)
4 protocols using 1 2 dioleoyl sn glycerol 3 phosphocholine
1
Lipid-based Nanoparticle Preparation Protocol
2
Tethering Anti-Ig on Planar Lipid Bilayers
3
Lipid-Peptide Nanoparticle Synthesis
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1,2-dioleoyl-sn-glycerol-3-phosphocholine (DOPC), cholesterol, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000 (DSPE-PEG2000) and DSPE-PEG3400-Maleimide were purchased from Avanti polar lipids, Miami, FL. 1,1'-dioctadecyl-3,3,3'3'-tetramethylindotricarbocyanine iodide (DiR) was purchased from Invitrogen, Carlsbad, CA. Peptides were synthesized by the Tufts University peptide synthesis core facility using standard FMOC chemistry and Rink-Amide resin (Tufts University, Boston, MA).
4
Antigen Immobilization on Planar Lipid Bilayers and Plasma Membranes
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PLB was prepared in 8-well Lab-Tek chamber (#1.0 Borosilicate coverglass system, Nunc) as described before (29 (link)). Briefly, PLB was prepared using 110 μM small unilamellar vesicles consisting of 1,2-dioleoyl-sn-glycerol-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (DOPE-cap biotin) (Avanti Polar Lipids) in ratio 100:1. To bind Ag to the PLB, the wells containing PLB were incubated at RT with 2.5 μg/ml streptavidin for 10 min, followed by 1 μg/ml biotinylated goat F(ab′)2 anti-human λ + κ (Southern biotech) for 20 min.
PMS was prepared in 8-well Lab-Tek chamber (#1.5 Borosilicate coverglass system, Nunc) as previously described (30 (link)). Briefly, 293A cells (1 × 105) were seeded in poly-l-lysine-coated wells and cultured overnight in complete RPMI at 37°C, 80–90% confluency being achieved. Cells were washed with HBSS and sonicated with a probe sonicator (5 s, 22% power) in HBSS containing 2% BSA to obtain PMS. To bind Ag to the PMS, the wells containing PMS were first blocked with HBSS containing 2% BSA for 30 min at RT and incubated sequentially for 30 min with 1 μg/ml biotinylated annexin V (Biolegend), 1 μg/ml streptavidin and 0.5 μg/ml biotinylated goat F(ab′)2 anti-human λ + κ (Southern biotech).
Ag concentrations for PLB and PMS were selected by titration measurements to contain the same amounts.
PMS was prepared in 8-well Lab-Tek chamber (#1.5 Borosilicate coverglass system, Nunc) as previously described (30 (link)). Briefly, 293A cells (1 × 105) were seeded in poly-l-lysine-coated wells and cultured overnight in complete RPMI at 37°C, 80–90% confluency being achieved. Cells were washed with HBSS and sonicated with a probe sonicator (5 s, 22% power) in HBSS containing 2% BSA to obtain PMS. To bind Ag to the PMS, the wells containing PMS were first blocked with HBSS containing 2% BSA for 30 min at RT and incubated sequentially for 30 min with 1 μg/ml biotinylated annexin V (Biolegend), 1 μg/ml streptavidin and 0.5 μg/ml biotinylated goat F(ab′)2 anti-human λ + κ (Southern biotech).
Ag concentrations for PLB and PMS were selected by titration measurements to contain the same amounts.
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