Peripheral venous blood samples were collected within 7 days before the first round of NACT. PNI is calculated as serum ALB (g/L) + 5 × total lymphocyte count (109/L). Hematologic parameters were analyzed by an XE-2100 hematology analyzer (Sysmex, Kobe, Japan).
Xe 2100 hematology analyzer
Manufactured by Sysmex
Sourced in Japan, United States
The XE-2100 is a hematology analyzer designed for clinical laboratories. It is capable of performing complete blood count (CBC) and differential white blood cell (WBC) analysis. The XE-2100 utilizes flow cytometry technology to analyze and enumerate various blood cell types. It provides accurate and reliable results to support clinical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
Vacutainer, by BD (2 mentions)
Au5400 automatic chemical analyzer, by Olympus (2 mentions)
Elecsys e 602, by Roche (2 mentions)
Cobas c8000, by Roche (2 mentions)
Pathromtin, by Siemens (1 mentions)
Vwf ag, by Siemens (1 mentions)
Sodium citrate tube, by BD (1 mentions)
Hplc method, by Bio-Rad (1 mentions)
Enspire multiplate reader, by PerkinElmer (1 mentions)
Prostaglandin e2 parameter assay kit, by R&D Systems (1 mentions)
Cobas c501, by Roche (1 mentions)
Paxgene blood rna system, by Qiagen (1 mentions)
Xn 9000, by Sysmex (1 mentions)
Cobas e601 immunology analyzer, by Roche (1 mentions)
Ie33 echocardiography system, by Philips (1 mentions)
Au5800 system, by Beckman Coulter (1 mentions)
Cs 5100 system, by Sysmex (1 mentions)
Elx800, by Agilent Technologies (1 mentions)
Au680 biochemistry analyzer, by Beckman Coulter (1 mentions)
Automatic analyzer 7600, by Hitachi (1 mentions)
43 protocols using xe 2100 hematology analyzer
1
Peripheral Blood Sample Collection
2
Hematological Analysis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were sacrificed as described above, and blood was collected from the eyeball. Blood routine tests, including white blood cell (WBC), red blood cell (RBC) and blood platelet (PLT) counts were conducted using an XE-2100 hematology analyzer (Sysmex Corporation).
3
Stroke Risk Factors and Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Demographics (sex and age) and clinical data (glasgow coma scale score, history of hypertension, diabetes mellitus, smoking, alcohol consumption, coronary heart disease, cerebral infarction, and cerebral hemorrhage) were reviewed from the inpatient medical records. The time from symptom onset to baseline CT was also calculated. We collected laboratory parameters from the emergency medical record system, including glucose level, platelet count, international normalized ratio, total WBC count, and differential leukocyte counts (neutrophil count, monocyte count, lymphocyte count, and eosinophil count). Routine blood sampling for each participant was completed within 1 h after admission, but prior to the baseline CT imaging. Before testing, venous blood sample was placed in a 2.0 mL of disposable collection tube, which contained EDTA-K2 for the purpose of anticoagulation. Complete blood count was measured by an automatic XE-2100 hematology analyzer (Sysmex Corporation, Kobe, Japan).
4
Blood Coagulation Biomarker Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected from the antecubital vein into 0.109 mol/l trisodium citrate tubes (Vacutainer, Becton Dickinson, New Jersey, USA) in the morning, after an overnight fasting. Plasma samples were obtained by centrifuging blood at 2000 Â g for 15 min at room temperature for von Willebrand factor (vWF)-ag, fibrinogen, FVIII and at 4 8C only for PAI-1 evaluation. Complete blood cell count was performed by using the Sysmex XE-2100 hematology analyzer (Sysmex, Kobe, Japan). Fibrinogen was assessed by Clauss clotting method (Siemens, Marburg, Germany). Plasma PAI-1 antigen levels were measured with commercially available ELISA kits (PAI-1 Actibind ELISA, Technoclone, Wien, Austria). FVIII activity was determined by a coagulation-based assay, with deficient plasma in the presence of Pathromtin (Coagulation Factor VIII, Siemens); vWF antigen levels were detected by a turbidimetric assay (vWFAg, Siemens).
5
Blood Collection and Analysis Protocol
6
Comprehensive Metabolic Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Baseline blood samples were drawn after at least 10 h of fasting, and the collected samples were centrifuged at 1006 g for 10 min at 4 °C. Plasma glucose level was analyzed by the hexokinase method, while high-density lipoprotein (HDL)-cholesterol and low-density lipoprotein (LDL)-cholesterol levels were measured by homogeneous enzymatic assays, and triglyceride level was measured by a glycerol-3-phosphate oxidase peroxide method. An XE-2100 Hematology Analyzer (Sysmex Corp., Kobe, Japan) was used to measure white blood cell count, hemoglobin level, hematocrit level, and platelet count. Aspartate and alanine aminotransferase (NADH-UV method), total bilirubin (bilirubin oxidase method), blood urea nitrogen (urease/glutamate dehydrogenase method), creatinine (Jaffe’s kinetic method) levels were measured at the central laboratory of SNUBH. The biochemical tests were performed immediately after sample collection. Plasma insulin and C-peptide were measured by radioimmunoassay (Linco St. Louis, MO, USA), and HbA1c was measured using a HPLC method as reported previously (Bio-Rad, Hercules, CA, USA) [18 (link)].
7
Hematological and Biochemical Analyses of Murine Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peripheral blood was collected by both retro-orbital sinus bleeding and facial vein sampling under anesthesia. For hematological analysis, 200 μL of blood samples were collected in 1.5 mL tubes precoated with 50 μL of 50 UI/mL sodium heparin (Hospira Italia SrL, Napoli, Italy). The samples were then diluted at 1:1 with a saline solution and analyzed using an XE-2100 hematology analyzer (Sysmex, København S, Denmark) within 12 h of sampling. For biochemical analyses, 500 μL of blood was collected in 1.5 mL tubes and kept for 30 min at room temperature to form clots. Samples were centrifuged at 3200 rpm for 15 min, and the supernatant serum was transferred to new tubes and stored at −20 °C. The thawed sera were analyzed for creatinine dosage and aspartate transaminase (AST) activity (from whole sera and diluted 1:1 sera, respectively) with a spectrophotometer (Cobas C501, Roche Diagnostic). In addition, PGE2 levels were analyzed with a Prostaglandin E2 Parameter Assay Kit (R&D system) to quantify their levels in AFT and AFT-AD-MSC sera. The optical density at 450 nm was measured using an Enspire multiplate reader (PerkinElmer).
8
Fasting Blood Sample Collection and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We instructed the participants not to eat food after midnight. Baseline blood samples were drawn after at least 10 hours of fasting, and the collected samples were centrifuged at 3,000 rpm for 10 minutes at 4°C. Plasma glucose level was analyzed by the hexokinase method, while high-density lipoprotein (HDL) cholesterol and low-density lipoprotein cholesterol levels were measured by homogeneous enzymatic assays, and triglyceride level was measured by a glycerol-3-phosphate oxidase peroxide method. An XE-2100 Hematology Analyzer (Sysmex Corp., Kobe, Japan) was used to measure white blood cell count, hemoglobin level, hematocrit level, and platelet count. Aspartate and alanine aminotransferase (NADH-UV method), total bilirubin (bilirubin oxidase method), thyroid function (electrochemiluminescence immunoassay), blood urea nitrogen (urease/glutamate dehydrogenase method), creatinine (Jaffe’s kinetic method), calcium (o-cresolphtalein chromogenic method), and phosphorus (molybdate reduction method) levels were measured at the central laboratory of SNUBH. The biochemical tests were performed immediately after sample collection. Aliquots of serum, plasma, whole blood, DNA, and RNA (PAXgene Blood RNA System, Qiagen, Hilden, Germany) were kept in deep freezers until tested (−80°C).
9
Cardiovascular Risk Factors and Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Demographic data (age and sex) and history of risk factors (hypertension, diabetes mellitus, congestive heart failure, history of vascular disease, systolic blood pressure; diastolic blood pressure, smoking, and alcohol abuse) were collected at admission via in-person interviews with the patients or their family members. Blood samples of patients were obtained in the next morning of the day of admission. After centrifugation, aliquots of the samples were immediately stored at –80 °C before assay. Routine blood biomarkers, including triglyceride, cholesterol, high-density lipoproteinm (HDL), low-density lipoprotein (LDL), lipoprotein (a), high-sensitivity C-reactive protein (hs-CRP), fasting blood glucose (FBG), albumin and Creatinine (Cr), were examined using standard detection methods. MPV as well as leukocyte, erythrocyte, and platelet counts were tested by a Sysmex XE-2100 hematology analyzer (Sysmex, Kobe, Japan). We collected echocardiographic parameters from all patients according to the current guidelines,[21 (link)] including left ventricular ejection fraction (LVEF), Left atrial diameter (LAD), left ventricular end-diastolic diameter (LVEDd), left ventricular end-systolic diameter (LVEDs), and inter-ventricular septal thickness at end diastole (IVSd).
10
Platelet-rich Plasma Preparation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This study was reviewed and approved by the Boston Children’s Hospital Committee on Clinical Investigation and all subjects provided written informed consent. Healthy volunteers were qualified for enrollment if they were aged ≥18 years, free of aspirin or other antiplatelet medication (≥10 days), and free of all other non-steroidal anti-inflammatory drugs (≥ 3 days). Blood, 120 mL, was collected into 1/10th volume of acid citrate dextrose (ACD) and PRP was prepared Harvest SmartPreP2 System (Harvest Technologies, Plymouth, MA, USA) according to the manufacturer’s recommendation as previously described [37 ]. Complete blood cell counts were performed in a Sysmex XE-2100 Hematology Analyzer. Prepared PRP had 1095.2 ± 192.9 x 109 platelets/L, 1.65 ± 0.26 ×1012 RBC/L, and 13.77 ± 3.98 x 109 WBC/L (mean ± SD).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!