Antioxidant assay

Manufactured by Cayman Chemical

The Antioxidant Assay is a laboratory tool designed to measure the antioxidant capacity of samples. It provides a quantitative analysis of the ability of a substance to neutralize free radicals or reactive oxygen species.

Automatically generated - may contain errors

Lab products found in correlation

Thiobarbituric acid reactive substances assay kit, by Cayman Chemical (1 mentions) Gsh gssg glo, by Promega (1 mentions) Dy453, by R&D Systems (1 mentions) Modulus 2 microplate multimode reader, by Promega (1 mentions) Duoset elisa kit, by R&D Systems (1 mentions) Dy479, by R&D Systems (1 mentions) Celltiter glo kit, by Promega (1 mentions)

2 protocols using antioxidant assay

Antioxidant Capacity in Kidney Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antioxidant Assay (Cayman Chemical, Item No. 709001) was used to measure the total antioxidant capacity of kidney cell lysates. Kidney tissue (∼10–15 mg/sample) from each studied group was sonicated in the assay buffer on ice, then cells were spinned down by centrifugation (10,000 × g for 15 min at 4°C). The supernatant was diluted 1:50 and used for further analysis according to the manufacturer’s instructions. Absorbance was measured in 96-well plates with a Biotek Instruments plate reader at 405 nm. The capacity of the antioxidant system was compared with a standard curve obtained with Trolox (water-soluble tocopherol analog) and quantified as milliMolar (mM) Trolox equivalents.

Domondon M., Polina I., Nikiforova A.B., Sultanova R.F., Kruger C., Vasileva V.Y., Fomin M.V., Beeson G.C., Nieminen A.L., Smythe N., Maldonado E.N., Stadler K, & Ilatovskaya D.V. (2020). Renal Glomerular Mitochondria Function in Salt-Sensitive Hypertension. Frontiers in Physiology, 10, 1588.

+ Open protocol

+ Expand

Cardiac Metabolic and Oxidative Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

ATP content was measured in whole cardiac tissue homogenate using the CellTiter‐GLO kit (G7570, Promega). Glutathione as well as total antioxidant capacity were determined in homogenates by the GSH/GSSG‐GLO (V6611, Promega) and Antioxidant Assay (709001, Cayman Chemicals) kits, respectively. As an index of ROS, lipid peroxidation levels were assessed using a thiobarbituric acid reactive substances assay kit (10009055, Cayman Chemicals). Measurement of the chemokines C‐C motif chemokine ligand 2 (CCL2) and C‐X‐C motif chemokine ligand 1 (CXCL1) in cardiac lysates was achieved using the CCL2/JE/MCP1 (DY479, R&D Systems) and CXCL1/KC (DY453, R&D Systems) DuoSet ELISA kits, respectively. All assays were performed according to the manufacturer's instructions in a 96‐well plate. Data were acquired using a Modulus II microplate multimode reader (Promega).

Marshall K.D., Klutho P.J., Song L., Roy R., Krenz M, & Baines C.P. (2023). Cardiac Myocyte‐Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 12(4), e025867.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!