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2 protocols using rabbit anti snap23

1

Multimodal Protein Detection Assay

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Rabbit anti-SNAP23, -Syntaxin4, -VAMP8, –Actin and mouse anti-alpha tubulin were purchased from Sigma. Rabbit anti-VAMP2 was obtained from Synaptic Systems. Anti-p80 (clone 5G10, mouse) and –phospho-SNAP23 (Serine 120, rabbit) were kind gifts from Drs. J. Bonifacino and P. Roche, respectively. Anti-IKKβ (rabbit) was purchased from Cell Signaling and anti-FcεRIα (mouse) was obtained from Abcam. Mouse anti-SNAP23 and -phospho IκBα were purchased from Santa Cruz Biotechnology. HRP-conjugated Cholera toxin subunit B and Goat anti-mouse IgG1-AlexaFluor488 conjugated antibody was obtained from Invitrogen. Sheep anti-mouse and donkey anti-rabbit HRP-conjugated antibodies were purchased from GE Healthcare.
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2

Immunofluorescence Staining for Intracellular Proteins

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The following primary antibodies were used: rabbit anti-IncA (gift from T. Hackstadt), goat anti-MOMP (Virostat), rabbit anti-Syntaxin 3 (Synaptic Systems), rabbit anti-Syntaxin 4 (Sigma), rabbit anti-SNAP-23 (Sigma), mouse anti-alpha tubulin (Sigma), mouse anti-FLAG (Sigma, M2), chicken anti-HSP70 (StressMarq). Goat anti-rabbit IgG-Alexa Fluor 488, goat anti-rabbit IgG-Alexa Fluor 647, goat anti-mouse IgG-Alexa Fluor 488, goat anti-rabbit IgG-Alexa Fluor 555, donkey anti-goat IgG-Alexa Fluor 488, donkey anti-rabbit IgG-, anti-mouse IgG-, anti-goat IgG-, and anti-chicken IgY-horseradish peroxidase (HRP)-conjugated secondary antibodies, Hoechst, and NBD-C6-ceramide were purchased from Invitrogen. BODIPY 493/503 and CFSE Cell Proliferation Kit were purchased from ThermoFisher.
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