Rabbit and mouse horseradish peroxidase conjugated secondary antibody

Manufactured by Cell Signaling Technology

Rabbit and mouse Horseradish-peroxidase-conjugated secondary antibody is a laboratory reagent used to detect and visualize primary antibodies in various immunochemical techniques, such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISAs). The secondary antibody is conjugated with the enzyme horseradish peroxidase, which catalyzes a colorimetric or chemiluminescent reaction when exposed to its substrate, enabling the visualization of the target protein.

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Lab products found in correlation

2 protocols using rabbit and mouse horseradish peroxidase conjugated secondary antibody

Immunodetection of HSPB8 and autophagy

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Mouse anti-HSPB8 (MAB4987) was from R&D System-Biotechne (Minneapolis, MN, USA). Rabbit anti-HSPB8 (PA5-76780) and rabbit anti-SQSTM1/p62 (PA5-20839) were from ThermoFisher Scientific (Waltham, MA, USA). Rabbit anti-E-cadherin (#3195), rabbit anti-N-cadherin (#13116), rabbit anti-vimentin (#5741), rabbit anti-ERK1/2 (#4695), rabbit anti-pERK1/2 (#4370), rabbit anti-cyclin D (#2926), rabbit anti-CDK4 (#12790), rabbit anti-Akt1 (#2938), rabbit anti-pAkt (Ser473) (#9271), rabbit anti-mTOR (#2983), rabbit anti-p-mTOR (Ser 2448) (#5536), rabbit anti-ATG5 (#12994) were from Cell Signaling (Danvers, MA, USA). Rabbit anti-BRAFV600E (RM8) was from RevMAb Biosciences (San Francisco, CA, USA). Mouse anti-pan-RAS (C-4) (sc-166691) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-LC3 (L8918) and mouse anti-alpha-tubulin (T6199) were from Sigma–Aldrich (St. Louis, MO, USA). Rabbit and mouse Horseradish-peroxidase-conjugated secondary antibody were from Cell Signaling. 3-Methyladenine (3-MA) (S2767) was from Selleckchem (Munich, Germany). Chloroquine (CQ) (C6628) was from Sigma–Aldrich.

Cristofani R., Piccolella M., Montagnani Marelli M., Tedesco B., Poletti A, & Moretti R.M. (2022). HSPB8 counteracts tumor activity of BRAF- and NRAS-mutant melanoma cells by modulation of RAS-prenylation and autophagy. Cell Death & Disease, 13(11), 973.

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Autophagy Evaluation in Cell Lines

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Rabbit anti-LC3 (L8918), mouse anti-alpha-tubulin (T6199) and mouse anti-FLAG^® clone M2 (F1804) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-SQSTM1/p62 (PA5-20839) was from ThermoFisher Scientific (Waltham, MA, USA). Rabbit anti-procaspase-3 (#9665), rabbit anti-cleaved-caspase-3 (Asp-175, clone 5A1E) (#9664), mouse anti-procaspase-9 (#9508), rabbit anti-PARP (#9542), rabbit anti-cleaved-PARP (#5625), rabbit anti-Atg5 (#12964) were from Cell Signaling (Danvers, MA, USA). Mouse anti-cytochrome c (7H8) (sc-13560) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-GFP (Ab1218) was from Abcam (Cambridge, UK). Rabbit and mouse horseradish-peroxidase-conjugated secondary antibody were from Cell Signaling. FITC-conjugated secondary antibody Alexa Fluor 488, MitoTracker Orange CMTM Ros (mitochondrial selective dye) (M7510) and the LysoTracker Green DND-26 (lysosomal selective dye) (L7526) were acquired from Molecular Probes (ThermoFisher Scientific). Trehalose (T0167), rapamycin (R0395), chloroquine (CQ) (C6628), ammonium chloride (NH₄Cl) and docetaxel were purchased from Sigma-Aldrich. 3-MA (S2767) was obtained from Selleckchem (Munich, Germany).

Cristofani R., Montagnani Marelli M., Cicardi M.E., Fontana F., Marzagalli M., Limonta P., Poletti A, & Moretti R.M. (2018). Dual role of autophagy on docetaxel-sensitivity in prostate cancer cells. Cell Death & Disease, 9(9), 889.

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