129s parp1tm1zqw j

Parp1-floxed mice were provided by Dr. Kraus (Luo et al., 2017 (link)) via an approved Material Transfer Agreement (MTA). Olig2-Cre (B6.129-Olig2^{tm1.1(cre)Wdr}/J, stock 025567), Pdgfrα-CreER^T2 (B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J, stock 018280) and Rosa26-LoxP-STOP-LoxP-EYFP (referred to as Rosa26-EYFP, B6.129X1-Gt(ROSA)26Sor^tm1(EYFP)Cos/J, stock 006148) were purchased from the Jackson Laboratory. Cre transgene was always maintained as heterozygosity. We crossed Cre lines with Parp1^fl/fl mice to generate Parp1 cKO mice. Mice that are homozygous for Parp1 KO were purchased from the Jackson Laboratory (129S-Parp1^tm1Zqw/J, stock 002779) and bred with C57BL/6J mice (stock 002779, Jackson Laboratory) to get heterozygous Parp1 KO mice. WT and Parp1 KO mice were subsequently generated by crossing female heterozygous Parp1 KO mice with male heterozygous Parp1 KO mice. Both male and female mice were used in this study. All animals were from C57BL/6 background and maintained in 12 h light/dark cycle with water and food. Animals and procedures in this study were approved by the Institutional Animal Care and Use Committee at the University of California, Davis.

Mice with a homozygous deletion of Parp1 (129S-Parp1^tm1Zqw/J, referred to henceforth as Parp1^−/−) (40 (link)) and the corresponding 129S wild-type strain were purchased from the Jackson Laboratory (Bar Harbor, ME) and bred at Georgetown University (Washington, DC). All mouse work was conducted at Georgetown University under Protocol 13-003 approved by the Georgetown University Institutional Animal Care and Use Committee, and in accord with NIH guidelines. The animals had access to food and water ad libitum and were maintained with a standard 12 h light/dark cycle.
Male mice at 8–10 weeks of age were exposed to approximately LD_50/30¹³⁷Cs gamma rays, corresponding to 6 Gy for the Parp1^−/− animals (36 (link)) and 8.8 Gy for the WT mice (37 (link)). The dose rate was approximately 1.67 Gy/min. A second group of WT animals were also exposed to 6 Gy, and for both genotypes a group of control animals were sham irradiated.
At 24 h postirradiation animals were sacrificed, and blood was collected by cardiac puncture into PAXgene™ blood RNA stabilization solution (PreAnalytiX; QIAGEN^®, Valencia, CA). After mixing thoroughly, the samples were stored at −80°C prior to shipping to Columbia University (New York, NY) for RNA analysis.