Mouse fitc conjugated anti cd105 antibody

When confluent, the MSCs were passaged 1 in 3, and a sample was analyzed for MSCs marker expression by flow cytometry. The cells were washed in phosphatebuffered saline (PBS), and then removed from the flask by 0.05% trypsin (Gibco). 1 × 10⁵ cells were incubated with each mouse monoclonal primary antibody at 4°C for 30 minutes. Mouse FITC–conjugated anti-CD105 antibody (1:100 dilution), mouse PE–conjugated anti-CD146 antibody (1:100 dilution), and mouse FITC–conjugated anti-CD34 antibody (1:100 dilution) were purchased from Beckton Dickinson (Oxford, UK). Mouse PE–conjugated anti-STRO-1 antibody (1:50 dilution) was purchased from Santa Cruz (CA, USA). After wash, the cells were resuspended in 500 μl wash buffer and analyzed on a BD flow cytometer (Oxford, UK).

When confluent, the MSCs were passaged 1 in 3, and a sample was analyzed by flow cytometry for MSC marker expression. The cells were washed in PBS and then removed from the flask by 0.05 % trypsin (Gibco). 1 × 10⁵ cells were incubated with each mouse monoclonal primary antibody at 4 °C for 30 min. Mouse FITC-conjugated anti-CD105 antibody (1:100 dilution) and mouse FITC-conjugated anti-CD34 antibody (1:100 dilution) were purchased from Becton Dickinson (Oxford, UK). Mouse FITC-conjugated anti-αSMA antibody (1:25 dilution) was purchased from Abcam (Cambridge, UK). Mouse PE-conjugated anti-STRO-1 antibody (1:50 dilution) was purchased from Santa Cruz (CA, USA). After wash, the cells were resuspended in 500 μL wash buffer and analyzed on a BD flow cytometer (Oxford, UK).