Small cubic pieces ≤ 1 mm3 were dissected from left ventricles and fixed with 2.5% glutaraldehyde in 0.1 mol/ L sodium phosphate (pH 7.4) overnight at 4 °C. After post-fixation in 1% OsO4, samples were dehydrated through graded alcohols and embedded in Epon Araldite. Ultrathin sections (50 nm) were cut using an ultramicrotome (Ultracut E, Leica), and stained with uranyl acetate and lead citrate. The specimens were viewed on a Hitachi H-7000 Electron Microscope (Pleasanton, CA, USA). Images were captured using a Gatan high resolution 4 k × 4 k digital camera and Gatan Ditital Micrograph software (22 (link)).
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Lab products found in correlation
2 protocols using high resolution 4 k 4 k digital camera
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Ultrastructural Analysis of Cardiac Tissue
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Ultrastructural Analysis of Cardiac Tissue
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Small cubic pieces ≤ 1 mm3 were dissected from left ventricles and fixed with 2.5% glutaraldehyde in 0.1 mol/ L sodium phosphate (pH 7.4) overnight at 4 °C. After post-fixation in 1% OsO4, samples were dehydrated through graded alcohols and embedded in Epon Araldite. Ultrathin sections (50 nm) were cut using an ultramicrotome (Ultracut E, Leica), and stained with uranyl acetate and lead citrate. The specimens were viewed on a Hitachi H-7000 Electron Microscope (Pleasanton, CA, USA). Images were captured using a Gatan high resolution 4 k × 4 k digital camera and Gatan Ditital Micrograph software (22 (link)).
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