Stock solutions of fractionated SBT-derived butanol extracts were prepared in 100% methanol (phenolic fractions) or 100% DMSO (lipid fractions). Further dilutions of each stock were prepared in a medium adapted to the requirements of the test. Reference compounds, such as epicatechin, ellagic acid (both from Roth, Karlsruhe, Germany), quercetin, and ursolic acid (both from Sigma, Steinheim Germany) were dissolved in 100% DMSO and diluted in the appropriate medium. In some parts of the manuscript and Supplementary Materials (Tables and Figures) , fractions were designated as LF, leaf/phenolic; LL, leaf/lipid; GF, twig/phenolic; GL, twig/lipid; OF, fruit/phenolic; OL, fruit/lipid.
Epicatechin
Manufactured by Carl Roth
Sourced in Germany
Epicatechin is a natural polyphenol compound that can be used as a reference standard in analytical laboratories. It is a flavanol found in various plant sources such as cocoa, green tea, and berries. Epicatechin is primarily used for identification and quantification purposes in analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Catechin, by Carl Roth (2 mentions)
Ursolic acid, by Merck Group (1 mentions)
Ellagic acid, by Carl Roth (1 mentions)
Quercetin, by Merck Group (1 mentions)
Chlorogenic acid, by Carl Roth (1 mentions)
2 2 azino bis 3 ethylbenzthiazoline 6 sulfonic acid diammonium salt, by Merck Group (1 mentions)
Aceton, by Avantor (1 mentions)
Quercetin 3 glucoside, by Carl Roth (1 mentions)
Trolox, by Merck Group (1 mentions)
Potassium dihydrogen phosphate, by Merck Group (1 mentions)
Nitric acid, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Carl Roth (1 mentions)
Folin ciocalteu s phenol reagent, by Merck Group (1 mentions)
Apigenin, by Cayman Chemical (1 mentions)
Gallic acid, by Carl Roth (1 mentions)
Ellagic acid, by Cayman Chemical (1 mentions)
Isorhamnetin 3 o glucoside, by Extrasynthese (1 mentions)
Hyperoside, by Cayman Chemical (1 mentions)
Kaempferol 3 o glucuronide, by Extrasynthese (1 mentions)
Quercetin 3 o glucuronide, by Extrasynthese (1 mentions)
4 protocols using epicatechin
1
Fractionated SBT-derived Butanol Extracts
2
Detailed Analytical Protocols for Polyphenol Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Disodium hydrogen phosphate dodecahydrate was purchased from Bernd Kraft GmbH (Duisburg, Germany). Sodium dihydrogen phosphate monohydrate was from AppliChem GmbH (Darmstadt, Germany) and catechol was from ThermoFisher GmbH (Kandel, Germany). Aceton was used from VWR International LLC (Fontenay-sous-Bois, France). The standards for the HPLC analysis (chlorogenic acid, catechin, epicatechin, phloretin-2-glucoside, and quercetin 3-glucoside) and hydrochloric acid (25%) were from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). Sodium carbonate was purchased from Grüssing GmbH (Filsum, Germany) and potassium peroxodisulphate was from Fisher Scientific UK Ltd. (Loughborough, UK). Folin-Ciocalteu’s phenol reagent, potassium dihydrogen phosphate, and nitric acid (65%) were purchased from Merck KgaA (Darmstadt, Germany). Galllic acid and 2,2’-azobis(2-methylpropionamidine) dihydrochloride were from Fisher Scientific GmbH (Schwerte, Germany). 2,2’-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt, trolox, and fluorescein were purchased from Sigma-Aldrich Chemie GmbH (Deisenhofen, Germany). All of the chemicals were of analytical grade. Water was purified through a Milli-Q water system (PURELAB®, Elga LabWater, Veolia Water Technologies GmbH, Celle, Germany) and was used for buffers, the extracting agents, and dilution of sample extracts.
3
Phytochemicals for Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The reference phytochemicals, including isorhamnetin 3-O-glucoside, kaempferol 3-O-glucuronide, and quercetin 3-O-glucuronide were obtained from Extrasynthese (Genay, France). Gallic acid, catechin, and epicatechin were obtained from Carl Roth (Karlsruhe, Germany). Procyanidin B1, procyanidin B2, procyanidin B3, and procyanidin C1 were purchased from Cayman Chemical (Ann Arbor, MI, USA), while agrimoniin, apigenin, 3-O-caffeoylquinic acid, ellagic acid, astragalin (kaempferol 3-O-glucoside), pedunculagin, avicularin (quercetin 3-O-arabinoside), hyperoside (quercetin 3-O-galactoside), isoquercitrin (quercetin 3-O-glucoside), and tiliroside (purity > 96%) were previously isolated in the Department of Pharmacognosy of Medical University of Białystok (Białystok, Poland) [22 (link),48 ,49 (link),50 (link),51 (link)]. All other analytical grade chemicals used in the study were obtained from Sigma-Aldrich (St. Louis, MO, USA). To obtain ultra-pure water, a POLWATER DL3-100 Labopol (Kraków, Poland) system was used. Investigated extracts (100 mg/mL) and 5-fluorouracil (50 mM) were dissolved in dimethyl sulfoxide (DMSO) to prepare stock solutions. Working solutions were prepared by dissolving stock solutions in a culture medium. The final concentration of DMSO in all working solutions used in the studies was 0.25%.
4
Antioxidant Potential of Raspberry Powder and Honey
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh raspberries (R. idaeus, cv. Remontant, from Domeniul Cerbi, Marginea, Suceava, Romania) and multifloral honey (RomHoney Group, Iași, Romania) were used in this work. The raspberries were dried by lyophilization and were ground to a fine powder using an electrical grinder. The following chemicals were used: pharmaceutical ethanol 96% (Chimopar Srl, Bucharest, Romania), D(+)-Glucose anhydrous extra pure, D(−)-Fructose, extra pure, D(+) Saccharose, reagent grade (Scharlau, Barcelona, Spain) Trolox 97% (Acros Organics, Thermo Fisher Scientific, Pittburghs, PA, USA), Gallic acid, 2,2-Diphenyl-1-picrylhydrazyl (Sigma-Aldrich, Merck Group, Darmstad, Germany), 2,2′-Azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid) diammonium salt, 98%, 2,4,6-tri (2-pyridyl-1,3,5-triazine) 98% (Alfa Aesar, Kandel, Germany), Folin Ciocalteu’s phenol reagent, Iron chloride (III) (Merck, Darmstadt, Germany), hydrochloric acid, acetic acid (Chimopar Srl, Bucharest, Romania), sodium acetate (Scharlau, Barcelona, Spain), HPLC standards: ferulic acid, p-coumaric acid, caffeic acid, quercetin dihydrate (Sigma-Aldrich, Merck Group, Darmstad, Germany), syringic acid, luteolin, (+)-rutin trihydrate, (Alfa Aesar, Haverhill, MA, USA), chlorogenic acid, myricetin (Cayman Chemical, Ann Arbor, MI, USA), apigenin, (−) epicatechin (Roth, Karlsruhe, Germany), and kaempferol (Cayman Chemical, Ann Arbor, MI, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!