Mouse anti 6x his tag

Manufactured by Abcam

Mouse anti-6X his-tag is a monoclonal antibody that specifically recognizes the 6X histidine tag, a commonly used protein tag for recombinant protein purification and detection.

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Lab products found in correlation

Rabbit anti parvalbumin, by Abcam (1 mentions) Hoechst, by Thermo Fisher Scientific (1 mentions) Chabc, by Merck Group (1 mentions) Biotinylated wfa, by Merck Group (1 mentions) Rabbit anti myc tag, by OriGene (1 mentions) Chicken anti neun, by Merck Group (1 mentions) Alexa fluor 647 conjugated goat anti rabbit igg, by Cell Signaling Technology (1 mentions) Rabbit anti cox 4 3e11, by Cell Signaling Technology (1 mentions) Alexa fluor 488 conjugated goat anti mouse igg, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Mouse anti-6X His tag antibody Mouse anti-6X His tag monoclonal antibody Anti-6X His tag Mouse anti-His-tag Anti-6X His Anti-His-tag His-tag Mouse anti-His Mouse anti

2 protocols using mouse anti 6x his tag

Comprehensive Neuronal Immunostaining Protocol

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For immunohistochemistry, virus injection, neuronal cultures and Western Blots, the following are used: goat anti-Nptx2 (Santa Cruz, sc-12125), chicken anti-NeuN (Millipore, abn91), rabbit anti-myc-tag (OriGene, TA100029), rabbit anti-parvalbumin (Abcam, ab11427), biotinylated WFA (Sigma-Aldrich, L1516), mouse anti-6X his-tag (Abcam, ab18184), Hoechst (Thermo Fisher, H3570), his-tag NPTX2 protein (R&D Systems, 10889), chABC (Sigma-Aldrich, C3667), test hyase (Sigma-Aldrich, H3506), strep hyase (Sigma-Aldrich, H1136), and lenti-cmv-Nptx2 lentivirus (10⁷ TU/mL, OriGene, MR206833L1V).

Van't Spijker H.M., Rowlands D., Rossier J., Haenzi B., Fawcett J.W, & Kwok J.C. (2019). Neuronal Pentraxin 2 Binds PNNs and Enhances PNN Formation. Neural Plasticity, 2019, 6804575.

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Immunofluorescence Staining of Subcellular Proteins

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Cells were fixed in 4% paraformaldehyde for 10 min and permeabilized 10 min with 0.1% Triton X-100. Immunofluorescence (IF) was performed following standard protocols and the following antibodies: rabbit anti-MGRN1 from Proteintech Group, (cat. #11285–1-AP, RRID: AB_2143351), mouse anti-6*XHIS tag from Abcam (cat. #ab7857, RRID: AB_2716573), rabbit anti-COX IV (3E11) from Cell Signaling Technology (cat. #4850, RRID: AB_2085424), Alexa Fluor 488 conjugated goat anti-mouse IgG (Cell Signaling Technology cat. #4412, RRID:AB_1904025) and Alexa Fluor 647 conjugated goat anti-rabbit IgG (Cell Signaling Technology cat. #4414, RRID: AB_10693544).

Gunn T.M., Silvius D., Lester A, & Gibbs B. (2019). Chronic and age-dependent effects of the spongiform neurodegeneration-associated MGRN1 E3 ubiquitin ligase on mitochondrial homeostasis. Mammalian genome : official journal of the International Mammalian Genome Society, 30(5-6), 151-165.

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