Zo 1 zo1 1a12

Manufactured by Thermo Fisher Scientific

The ZO-1 (ZO1-1A12) is a primary antibody used for the detection of the tight junction protein ZO-1 in various cell types. It is commonly used in immunocytochemistry, immunohistochemistry, and Western blotting applications to visualize the localization and expression of ZO-1.

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2 protocols using zo 1 zo1 1a12

Endotoxin-free HSP27 Biochemistry

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Low endotoxin rhHSP27 was purchased from Enzo Life Sciences (Villeurbanne, Fr) and rabbit anti-HSP27 from ABR (AffinityBioReagent, ThermoFisher, Fr). Recombinant human rhIL-8 and rhIL-7 were from R&D Systems. Mouse anti-Hsc70 was from Santa Cruz Biotech. Polymyxin B was from InvivoGen (Toulouse, Fr). Rabbit polyclonal anti-Cx43 (710700), mouse monoclonal anti-Cx43 (CX-1B1), anti-Cx32 (CX-2C2) and ZO-1 (ZO1-1A12) antibodies were from Invitrogen. Rabbit oligoclonal anti-Panx-1 (11HCLC) and rabbit polyclonal anti-CIP75 were from ThermoScientific (Rockford, USA) and anti-14-3-3, anti-phosphoserine and anti-CXCR2 from Abcam. DiL-C18, thapsigargin and fura-2/AM were from Molecular Probes. Other chemicals were from Sigma-Aldrich.

Thuringer D., Berthenet K., Cronier L., Solary E, & Garrido C. (2015). Primary tumor- and metastasis-derived colon cancer cells differently modulate connexin expression and function in human capillary endothelial cells. Oncotarget, 6(30), 28800-28815.

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Multicolor Immunohistochemistry of Brain Tissue

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Brian tissue sections were incubated overnight with primary antibodies at 4°C and then with corresponding fluorochrome-conjugated secondary antibodies at room temperature for 1 h. The following primary antibodies were used: anti-human NKp46 (195314; R&D Systems), CD49a (SR84; BD Bioscience), CD57 (MA1-81071; Invitrogen), CD68 (ED1; Abcam), CD4 (H-370; Santa Cruz), CD8 (UCH-T4; Santa Cruz), CD19 (HIB19; BioLegend), CD16b (CLB-gran11.5; BD Bioscience), CD66b (polyclonal; Bioss), perforin (dG9; BioLegend), CD69 (D-3; Santa Cruz), Caspase-3 (9661; CST), CD31 (JC/70A; Abcam); anti-mouse NKp46 (M20; Santa Cruz), CD49a (Ha31/8; BD Bioscience), ly6G (1A8; BioLegend), TMEM119 (28-3; Abcam), CD68 (ED1; Abcam), CD8 (53-6.7; eBioscience), CD4 (GK1.5; eBioscience), CD19 (1D3; BD Bioscience), CD31 (polyclonal; Abcam), claudin5 (4C3C2; Invitrogen), and ZO-1 (ZO-1-1A12; Invitrogen). The following fluorochrome-conjugated secondary antibodies were used: donkey anti-rabbit 488 (1:1,000; Invitrogen), donkey anti-rabbit 546 (1:1,000; Invitrogen), donkey anti-goat 546 (1:1,000; Invitrogen), donkey anti-mouse 594 (1:1,000; Invitrogen), donkey anti-mouse 488 (1:1,000; Invitrogen), goat anti-rat 488 (1:1,000; Invitrogen), and goat anti-rat 555 (1:1,000; Invitrogen). Images were acquired on a fluorescence microscope (Olympus BX-61).

Li Z., Li M., Shi S.X., Yao N., Cheng X., Guo A., Zhu Z., Zhang X, & Liu Q. (2020). Brain transforms natural killer cells that exacerbate brain edema after intracerebral hemorrhage. The Journal of Experimental Medicine, 217(12), e20200213.

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