Whole blood was transferred into serum separator tubes, allowed to clot, and centrifuged. Total cholesterol and triglycerides were determined by enzymatic procedures (Quest Diagnostics, Pittsburgh, PA; CV < 2% for both). HDL-C was estimated according to the modified heparin-manganese procedure (CV < 2%). LDL-C was not interpreted because calculated LDL-C is not accurate in the presence of ppTG. Insulin was measured by radioimmunoassay using 125I-labeled human insulin and a human insulin antiserum (Quest Diagnostics). Glucose was determined by an immobilized enzyme biosensor using the YSI 2300 STAT Plus Glucose & Lactate Analyzer (Yellow Springs Instruments, Yellow Springs, OH).
Ysi 2300 stat plus glucose lactate analyzer
Manufactured by Yellow Springs Instruments
Sourced in United States
The YSI 2300 STAT Plus Glucose & Lactate Analyzer is a laboratory instrument designed to measure the concentrations of glucose and lactate in a variety of samples. The device utilizes electrochemical detection methods to provide accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ysi 2300 stat plus glucose lactate analyzer
1
Blood Lipid and Glucose Analysis
2
Glucose, Insulin, and Insulin Resistance Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole blood glucose concentration was determined using the glucose oxidase method (YSI 2300 STAT PLUS Glucose & Lactate Analyzer, Yellow Springs Instruments, Ohio, USA). Serum insulin was measured with a commercially available enzyme-linked immunosorbent assay (EZHI-14K, Millipore Sigma). The homeostasis model assessment of insulin resistance 2 (HOMA2-IR) was used as an index of fasting insulin sensitivity [34 (link)].
3
Lipid and Glucose Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum and plasma aliquots from fasting blood samples were stored at −80°C until time of analysis. For the first 19 enrolled participants, samples were shipped frozen and analyzed in the core endocrine laboratory at the Milton S. Hershey Medical Center (Hershey, PA). Total cholesterol (TC) and triglycerides were measured by using enzymatic procedures with commercially available kits (Alfa Wassermann). HDL cholesterol was quantified according to the modified heparin-manganese precipitation procedure of Warnick and Albers (19 (link)). LDL cholesterol was calculated with the Friedewald equation: LDL cholesterol = TC – HDL cholesterol – (triglycerides ÷ 5) (20 (link)). Glucose was determined by an immobilized enzyme biosensor for glucose with the YSI 2300 STAT Plus Glucose & Lactate Analyzer (Yellow Springs Instruments). For the subsequent participants, lipids and glucose were measured in fresh samples by Quest Diagnostics by enzymatic procedures and spectrophotometry. The CVs for TC, HDL cholesterol, and triglycerides were <2%. For all participants, insulin was quantified by radioimmunoassay (Quest Diagnostics). Serum C-reactive protein was measured by latex-enhanced immunonephelometry (Quest Diagnostics; assay CV <8%).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!