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5 protocols using myricetin 3 galactoside

1

Identification of Polyphenol Compounds

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All the chemicals used in the experiments were of an analytical grade. Cyanidin chloride, cyanidin-3-galactoside, cyanidin-3-glucoside, cyanidin-3-arabinoside, peonidin chloride, peonidin-3-arabinoside, peonidin-3-glucoside, peonidin-3-galactoside, myricetin-3-galactoside, malvidin-3-galactoside, malvidin chloride, malvidin-3-arabinoside, delphinidin-3-galactoside (Extrasynthese, Genay, France), quercetin-3-O-glucoside (Biochemistry, Buchs, Switzerland), quercetin-3-galactoside, quercetin (Carl Roth, Karlsruhe, Germany), myricetin, quercetin-3-rhamnoside, quercetin-3-α-l-arabinofuranoside, quercetin-3-α-l-arabinopyranoside, oleanolic acid, ursolic acid, α-Amyrin, β-Amyrin, corosolic acid, and maslinic acid (Sigma-Aldrich, Steinheim, Germany) were used as standards for the identification of the compounds. Acetone, methanol, acetonitrile, hydrochloric acid, and 4-(Dimethylamino) cinnamaldehyde) were purchased from Sigma-Aldrich (Steinheim, Germany). Ethanol 96% (v/v) was purchased from AB Stumbras (Kaunas, Lithuania). Formic acid was bought from Merck (Darmstadt, Germany).
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2

Phytochemical Analysis of Medicinal Plants

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All solvents, reagents, and standards were of analytical reagent grade. Acetone, methanol, acetonitrile, hydrochloric acid, 4-(Dimethylamino) cinnamaldehyde and reference standarts for β-Amyrin, maslinic acid, oleanolic acid, corosolic acid, ursolic acid, α-Amyrin, quercetin-3-rhamnoside, myricetin, quercetin-3-α-L-arabinopyranoside and quercetin-3-α-L-arabinofuranoside were purchased from Sigma-Aldrich (Steinheim, Germany). Ethanol 96% (v/v) was purchased from AB Stumbras (Kaunas, Lithuania), formic acid was bought from Merck (Darmstadt, Germany), and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was purchased from Scharlau (Sentmenat, Spain). Reference standards for myricetin-3-galactoside, cyanidin chloride, peonidin chloride, malvidin-3-galactoside, malvidin-3-arabinoside, malvidin chloride, peonidin-3-arabinoside, cyaniding-3-galactoside, delphinidin-3-galactoside, peonidin-3-glucoside, peonidin-3-galactoside, cyaniding-3-glucoside and cyaniding-3-arabinoside were purchased from Extrasynthese (Genay, France). Standards for quercetin and quercetin-3-galactoside were purchased from Carl Roth (Karlsruhe, Germany). The standard for quercetin-3-O-glucoside was bought from Biochemistry (Buchs, Switzerland). Purified deionized water was provided using a water purification system (Milli-Q®, Millipore, Bedford, MA, USA).
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3

Comprehensive Phytochemical Analysis of Natural Compounds

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All reagents, standards, and solvents were of analytical reagent grade. Ethanol 96% (v/v) was bought from AB Stumbras (Kaunas, Lithuania). Solvents (acetone, methanol, and acetonitrile), reagents (hydrochloric acid and 4-(dimethylamino) cinnamaldehyde), and reference standards (myricetin, quercetin-3-rhamnoside, quercetin-3-α-L-arabinofuranoside, quercetin-3-α-L-arabinopyranoside, oleanolic acid, β-amyrin, corosolic acid, maslinic acid, α-amyrin, and ursolic acid) were bought from Sigma-Aldrich (Steinheim, Germany). Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was purchased from Scharlau (Sentmenat, Spain). Formic acid was purchased from Merck (Darmstadt, Germany). The standard for quercetin-3-glucoside was purchased from Biochemistry (Buchs, Switzerland). Standards for quercetin-3-galactoside and quercetin were bought from Carl Roth (Karlsruhe, Germany). Reference standards for peonidin chloride, cyanidin chloride, myricetin-3-galactoside, malvidin-3-galactoside, malvidin chloride, malvidin-3-arabinoside, cyaniding-3-galactoside, peonidin-3-arabinoside, peonidin-3-glucoside, delphinidin-3-galactoside, cyaniding-3-glucoside peonidin-3-galactoside, and cyaniding-3-arabinoside were bought from Extrasynthese (Genay, France).
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4

Chromatographic Analysis of Polyphenols

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Acetonitrile, formic acid, and methanol (MeOH) were of HPLC-gradient grade and were purchased from Panreac (Barcelona, Spain). The water was treated in a Milli-Q system (Millipore, Milford, MA, USA). Catechin, epiCatechin, Catechin gallate, epiCatechin gallate, procyanidins B1 and B2, myricetin-3-glucoside, myricetin-3-galactoside, querce-tin-3-glucoside, quercetin-3-glucuronide, kaempherol-3-glucoside, kaempherol-3-rutinoside, isorhamnetin-3-glucoside, and isorhamnetin-3-rutinoside were purchased by Extrasynthese (Lyon, France) and gallic, caffeic, ferulic, and coutaric acids were purchased from Aldrich (Munich, Germany).
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5

HPLC Analysis of Phenolic Compounds

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Analytical and chromatographic grade chemicals and solvents were used for this study: ethanol 96% (v/v) (manufacturer AB Stumbras, Kaunas, Lithuania), acetonitrile (manufacturer Sigma-Aldrich, Steinheim, Germany), methanol (manufacturer Sigma-Aldrich, Steinheim, Germany), and formic acid (manufacturer Merck, Darmstadt, Germany). Reference HPLC standards for chlorogenic acid (3-caffeoylquinic acid), myricetin, quercetin-3-rhamnoside, quercetin-3-α-L-arabinofuranoside, and quercetin-3-α-L-arabinopyranoside were obtained from Sigma-Aldrich (Steinheim, Germany), for quercetin-3-galactoside and quercetin—from Carl Roth (Karlsruhe, Germany), for myricetin-3-galactoside—from Extrasynthese (Genay, France), and the reference standard for quercetin-3-O-glucoside was obtained from Biochemistry (Buchs, Switzerland).
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