Anti mica

Manufactured by Abcam

Sourced in United States

Anti-MICA is a laboratory reagent that can be used to detect and quantify the presence of MICA (Major Histocompatibility Complex class I-related chain A) in biological samples. MICA is a stress-induced antigen that plays a role in the immune response. This product can be utilized in various immunological and cell biology research applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti rabbit cy3, by Jackson ImmunoResearch (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti mica, by R&D Systems (1 mentions) Anti ulbp 2 5 6, by R&D Systems (1 mentions) Anti flag tag, by BioLegend (1 mentions) Anti ulbp1, by R&D Systems (1 mentions) Anti ulbp3, by R&D Systems (1 mentions) Anti micb clone 236511, by R&D Systems (1 mentions) Anti vinculin, by Abcam (1 mentions) Mouse anti biotin, by Jackson ImmunoResearch (1 mentions) Anti mouse alexafluor 647, by Jackson ImmunoResearch (1 mentions) Streptavidin horseradish peroxidase hrp, by Jackson ImmunoResearch (1 mentions) Anti rat hrp, by Jackson ImmunoResearch (1 mentions) Streptavidin alexafluor 647, by Jackson ImmunoResearch (1 mentions) Anti β actin, by Merck Group (1 mentions) Ab59479, by Abcam (1 mentions) Anti micb, by Cell Signaling Technology (1 mentions) Anti muc1 c hm 1630 p1abx, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions) Bicinchoninic acid assay, by Thermo Fisher Scientific (1 mentions)

3 protocols using anti mica

Comprehensive Antibody Panel for Immunoanalysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used for flow cytometry: anti-MICA (clone 159227; R&D Systems), anti-MICB (clone 236511, R&D Systems), anti-ULBP1 (clone 170818; R&D Systems), anti-ULBP2/5/6 (clone 165903; R&D Systems) and anti-ULBP3 (clone 166514, R&D Systems).
The following primary antibodies were used for immunofluorescence: anti-PDI (ab3672, Abcam), anti-FLAG tag (Clone L5, Biolegend) and anti-MICA (clone 159227, R&D Systems).
The following primary antibodies were used for western blotting: anti-MICA (Clone EPR6568, Abcam), anti-FLAG tag (Clone L5, Biolegend), anti-GAPDH (clone 6C5, Santa Cruz) and anti-vinculin (clone EPR8185, Abcam).
The following secondary antibodies were used: anti-mouse AlexaFluor 647, anti-mouse PE, anti-mouse biotin, anti-rabbit biotin, anti-rat biotin, anti-rabbit Cy3, anti-rat 488, streptavidin-AlexaFluor 647, streptavidin-horseradish peroxidase (HRP), anti-mouse-HRP, anti-rat-HRP and anti-rabbit-HRP, all purchased from Jackson Laboratories.

Seidel E., Dassa L., Schuler C., Oiknine-Djian E., Wolf D.G., Le-Trilling V.T, & Mandelboim O. (2021). The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing. PLoS Pathogens, 17(5), e1008807.

+ Open protocol

+ Expand

Immunoblot Analysis of Cell Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein lysates from cultured cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:100 dilution; Thermo Fisher Scientific), anti-MICA (ab150355, 1:1000 dilution; Abcam), anti-MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich), anti-CD9 (13 174S, 1:1000 dilution; CST), anti-CD63 (ab59479, 1:1000 dilution; Abcam), anti-CD81 (56 039S, 1:1000 dilution; CST), anti-ERp5 (1:2500; 18 233–1-AP, Proteintech, Rosemont, Illinois, USA) and anti-RAB27A (69 295S, 1:1000 dilution; CST).

Morimoto Y., Yamashita N., Daimon T., Hirose H., Yamano S., Haratake N., Ishikawa S., Bhattacharya A., Fushimi A., Ahmad R., Takahashi H., Dashevsky O., Mitsiades C, & Kufe D. (2023). MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells. Journal for Immunotherapy of Cancer, 11(2), e006238.

+ Open protocol

+ Expand

Comprehensive Antibody Characterization for Western Blot and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: anti-MYCN, anti-p53, anti-Actin (B8.4.B, FL-393 and I-19, respectively, Santa Cruz Biotechnology), anti-MYC (Y69, OriGene), and anti-MDM2 (2A10, Calbiochem-Millipore) for western blotting; anti-CD107a-FITC (H4A3), anti-CD3-Alexa-700 (UCHT1), anti-CD56-PE-Cy7 (B159), anti-CD45 (HI30), FITC-conjugated rat anti-mouse IgG1 (A85–1) and PE-conjugated rat anti-mouse IgM (R6–60.2) purchased from BD Biosciences; anti-ULBP1-PE (170818), anti-ULBP2/5/6-PE (165903), anti-ULBP3-PE (166510), anti-MICA (159227), anti-MICB (236511), anti-TRAIL/R2-APC (17908), anti-CD155/PVR-PE (300907), anti-Nectin-2/CD112-APC (610603) purchased from R&D Systems; W6/32 which recognizes human fully-assembled MHC class I heavy chains and goat F(ab’)2 Fragment anti-mouse IgG FITC (IM1619, Dako) for flow cytometry; anti-MICA and anti-Nectin-2 (62540 and 154895, respectively, Abcam) for immunohistochemistry assay.
Whole-cell extracts were quantified by the bicinchoninic acid assay (Thermo Fisher Scientific), resolved on 8–10% SDS-PAGE and electroblotted. Filters were probed with primary antibodies followed by goat anti-mouse IgG HRP conjugated (Jackson). Flow cytometry was performed on FACSCantoII (BD Bioscences) and analyzed by FlowJo Software.

Brandetti E., Veneziani I., Melaiu O., Pezzolo A., Castellano A., Boldrini R., Ferretti E., Fruci D., Moretta L., Pistoia V., Locatelli F, & Cifaldi L. (2017). MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma. Oncoimmunology, 6(6), e1316439.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!