Anti tnf α blocking antibody

Manufactured by R&D Systems

Sourced in United States

The Anti-TNF-α blocking antibody is a laboratory reagent designed for use in research applications. It functions by binding to and neutralizing the activity of the pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α). This antibody can be used to study the role of TNF-α in various biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

TNF-α (1697 citations) Anti-TNF-α (30 citations) TNF-α antibody (5 citations)

2 protocols using anti tnf α blocking antibody

Isolation and Treatment of Murine Fibroblast-Like Synoviocytes and Chondrocytes

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

FLS were isolated from the joint synovium of wild-type and Il6-knockout (Il6^−/−) mice, as described previously.^{11 (link)} Subcultured FLS (between passages 4–8) were used for experiments. Flow cytometry was performed with antibodies against CD90 (a fibroblast marker) and CD14 (a macrophage marker) (Abcam, Cambridge, UK) to confirm the purity of our FLS (>90% CD90+/<1% CD14+). For isolation of chondrocytes, mouse cartilage tissues were isolated from the femoral condyles and tibial plateaus of wild-type and Il6^−/− mice, and chondrocytes were extracted by digestion with 0.2% collagenase type II, as described previously.^{10 (link), 16 (link)} Chondrocytes or FLS were treated with the indicated amounts of recombinant mouse IL-6 protein (Merck-Millipore, Billerica, MA, USA), recombinant mouse TNF-α protein (Merck-Millipore) or 800 MOI (multiplicity of infection) of empty adenovirus (Ad-C) or HIF-2α-expressing adenovirus (Ad-Epas1) for 24 h with or without subsequent treatment with the anti-TNF-α blocking antibody (R&D Systems Inc., Minneapolis, MN, USA).

Huh Y.H., Lee G., Song W.H., Koh J.T, & Ryu J.H. (2015). Crosstalk between FLS and chondrocytes is regulated by HIF-2α-mediated cytokines in arthritis. Experimental & Molecular Medicine, 47(12), e197-.

+ Open protocol

+ Expand

Treg Cell Suppression Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Treg cell suppression assays were performed as previously described.^{15 (link)} Briefly, Treg cells were depleted from PBMCs, and then Treg-depleted PBMCs were labeled with 5 μM carboxyfluorescein succinimidyl ester (CFSE) (Invitrogen). CFSE-labeled, Treg-depleted PBMCs, serving as responder T (Tresp) cells, were stimulated by soluble anti-CD3 antibody (0.1 μg/mL) and anti-CD28 antibody (1 μg/ml) with or without re-addition of the depleted Treg cell-enriched population with various ratios. Following 96 hours of co-culture, cells were analyzed for proliferation, as assessed by the percentage of CFSE^lo cells under either the live CD4⁺ or CD8⁺ T cell gates using an LSR II flow cytometer. Dead cells were excluded by staining cells with 7-AAD. The percentage of suppression was calculated as [1 - (% T cell proliferation with Treg cells/% T cell proliferation without Treg cells)] × 100.
To examine the effect of TNF-α on the suppression activity of Treg cells, anti-TNF-α blocking antibody (5 μg/ml; R&D Systems) or mouse IgG1 isotype control antibody was added to the co-cultures.

Choi Y.S., Jung M.K., Lee J., Choi S.J., Choi S.H., Lee H.W., Lee J.J., Kim H.J., Ahn S.H., Lee D.H., Kim W., Park S.H., Huh J.R., Kim H.P., Park J.Y, & Shin E.C. (2017). Tumor Necrosis Factor-producing T-regulatory Cells are Associated With Severe Liver Injury in Patients With Acute Hepatitis A. Gastroenterology, 154(4), 1047-1060.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!