Recombinant il 1β

A total of 200,000 cells were grown in 500 μL of growth medium per well in a 24-well plate. This growth medium includes 48% StemPro media with Pen-Strep (1%) as an antibiotic, as well as the following growth factors: human rEGF (recombinant epidermal growth factor) (20 ng mL⁻¹) (Biolegend, San Diego, CA, USA), human rGDNF (glial cell line-derived nerve growth factor) (10 ng mL⁻¹) (Biolegend), mouse rLIF (leukemia inhibitory factor) (10 ng mL⁻¹) (Biolegend), and mouse r-bFGF (basic fibroblast growth factor) (10 ng mL⁻¹) (Biolegend). The growth medium also included 10% KSR (Gibco, Grand Island, NY, USA) and 42% methylcellulose (R&D, Minneapolis, MI, USA). Recombinant IL-1β (Biolegend) in different concentrations (10, 100, 1000 pg/mL) was added according to the treatment protocol.