Hoechst 33258 cell apoptosis staining kit

Apoptosis was further determined by nucleus morphology using Hoechst 33258 cell apoptosis staining kit (Beyotime, Jiangsu, China). MCF‐7, MDA‐MB‐231, and Hs 578Bst were cultured in six‐well plates to 70% confluence, and then treated with various concentrations of deguelin for 48 h. After being fixed and stained with Hoechst 33258, the cells were examined under a Zeiss AxioImager A1 fluorescent microscope (Carl Zeiss).

A Hoechst 33258 cell apoptosis staining kit (Beyotime, Nanjing, China) was used to confirm morphological changes in the nuclei. The transfected cells were seeded onto sterile glass coverslips placed in 6-well plates and incubated for 24 h. The cells were fixed, washed twice with PBS, and stained with Hoechst 33258 staining solution for 5 min at room temperature in the dark. The cells were then washed twice with PBS, examined, and immediately photographed under a fluorescence microscope with an excitation wavelength of 350 nm. Apoptotic cells were defined by the condensation of nuclear chromatin or fragmentation to the nuclear membrane.