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8 protocols using benzeneacetaldehyde

1

Characterization of Aroma Compounds

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Standard 2‐hexanone, hexanal, 1H‐1ethyl‐pyrrole, 2‐hexenal, cis‐3‐hexen‐1‐ol, heptanal, 6‐methyl‐5‐hepten‐2‐one, 2‐pentyl‐furan, 2‐ethyl‐1‐hexanol, benzeneacetaldehyde, cis‐linalool oxide, translinalool oxide, linalool, 3‐octen‐2‐ol, phenylethyl alcohol, menthol, α‐terpineol, safranal, decanal, camphene, geraniol and indole were purchased from Sigma Co. Ltd. Benzyl alcohol, trans‐β‐damascenone, trans‐α‐ionone, cis‐geranylacetone, trans‐β‐ionone, 2,4‐ditert‐butylphenol, cedrol, and caryophyllene oxide were purchased from Alfa Aesar Co. Ltd. Standard chemical series of C8–C20 alkanes that were used to determine the liner retention index (RI) and the internal standard cyclohexanone were obtained from Sigma Co. Ltd. Cispyranoidlinalool oxide and transpyranoidlinalool oxide were purchased from Sinopharm Chemical Reagent Co. Ltd.
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2

Analytical Standards for Aroma Profiling

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The standards 2-hexanone, benzeneacetaldehyde, linalool, linalool oxides, phenylethyl alcohol, 4-oxoisophorone, menthol, α-terpineol, safranal, geraniol, indole, and 2-ethyl-3-methylpyrazine were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). The standards, 1-ethylpyrrole, benzyl alcohol, 1,2-dimethoxybenzene, 3,4-dimethoxytoluene, 1,2,3-trimethoxybenzene, 1,2,4-trimethoxybenzene, trans-β-ionone, 2,4-ditert- butylphenol, and dihydroactinidiolide were obtained from Alfa Aesar Co., Ltd. (Heysham, Lancashire, UK). A standard series of C8–C20 alkanes were used for retention index (RI) determination, and the internal standard cyclohexanone were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, Mo, USA). Other reagents were all of analytical grade and obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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3

Analysis of Volatile Compounds

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C7-C40 saturated alkane mixture, hexanal, (E)-2-hexenal, 1-hexanol, 1-octen-3-ol, β-myrcene, octanal, (E,E)-2,4-heptadienal, benzeneacetaldehyde, nonanal, (E,Z)-2,6-nonadienal, 2,6,6-trimethyl-2-cyclohexene-1,4-dione, decanal, α-ionone, and β-ionone were purchased from Sigma-Aldrich Company ltd., USA. Benzaldehyde and benzyl alcohol were from Aladdin Bio-Chem Technology Co., ltd, Shanghai, China. 2-acetyl-1-pyrroline was purchased from TRC (Canada).
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4

Comprehensive Protocol for Diverse Chemical Compounds

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2-Methyl-1-propanol, 3-methyl-1-butanol, 3-methyl-butanal, 3-octanone, p-xylene, styrene, benzeneacetaldehyde, phenylethyl alcohol, 4-ethyl-phenol, 2,5-dimethyl-pyrazine, trimethyl-pyrazine, and 3-ethyl-2,5-dimethyl-pyrazine were purchased from Sigma-Aldrich China Inc. (Beijing, China). The 18 amino acid standards, including glycine (Gly), asparagine (Asn), alanine (Ala), glutamic acid (Glu), lysine (Lys), arginine (Arg), proline (Pro), valine (Val), tyrosine (Tyr), isoleucine (Ile), leucine (Leu), tryptophan (Try), serine (Ser), histidine (His), threonine (Thr), methionine (Met), cysteine (Cys) and phenylalanine (Phe), were all purchased from Biosharp.
High-performance liquid chromatography (HPLC)-grade acetonitrile was supplied by Tedia Company (Fairfield, OH, USA). Phenylisothiocyanate (PITC, lot code: 10113227) was purchased from Alfa Aesar Chemical Co. Ltd (Tianjin, China), with a purity higher than 97%. Triethanolamine (TEA, analytical grade) and n-hexane (analytical grade) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Pure water was obtained using a Milli-Q purification system (Millipore, Bedford, MA, USA).
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5

Volatile Compound Profiling of Botanicals

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Standard 2-hexanone, 1H-1-ethyl-pyrrole, benzeneacetaldehyde, cis-linalool oxide, trans-linalool oxide, linalool, 3-octen-2-ol, phenylethyl alcohol, α-terpineol, safranal, camphene, geraniol, indole, 3-hexanol, hexanal, 1-octanol and 2,5-dimethylpyrazine were purchased from Sigma Co. Ltd. (St. Louis, MO, USA). Benzyl alcohol, trans-β-damascenone, trans-β-ionone, 2, 4-ditert-butylphenol, cedrol and caryophyllene oxide were purchased from Alfa Aesar Co. Ltd. (Heysham, Lancashire, UK). Standard chemical series of C8-C20 alkanes that were used to determine the liner retention index (RI) and the internal standard (Cyclohexanone) were obtained from Sigma Co. Ltd. (St. Louis, MO, USA). The other chemicals were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).
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6

Volatile Compounds in Duck Leg Meat

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Duck leg meat that was purchased from a local market (Xiamen, China) was stored at −20 °C in a refrigerator. Instant Oolong tea powder containing 25.07% (label provision) of tea polyphenols was purchased from Fujian Da Ming Co., Ltd. (Zhangzhou, China). A standard series of C8-C20 alkanes for retention index (RI) determination and the internal standard 2,4,6-trimethylpyridine (≥98%) and ethyl decanoate (≥98%) were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, USA). The standards, including 1-pentanol (≥98%), (Z)-3-hexen-1-ol (≥90%), benzaldehyde (≥97%), furfural (≥95%), (E)-2-hexenal (≥98%), benzeneacetaldehyde (≥98%), (E)-linalool oxide (≥96%), linalool (≥95%), phenylethyl alcohol (≥90%), 1-octen-3-ol (98%), (E)-2-nonenal (≥95%), (E,E)-2,4-heptadienal (≥98%), (E)-2-nonenal (≥98%), safranal (≥90%), decanal ≥(95%), β-cyclocitral (≥95%), 2,4-decadienal (≥90%), (E,E)-2,4-decadienal (≥95%), (E)-dodecanal (≥95%), alpha-terpineol (≥96%), hexanal (≥95%), β-ionone (≥97%), nonanal (≥96%), limonene (≥97%), dihydroactinidiolide (≥95%) and diisobutyl phthalate (≥98%) were purchased from Sigma-Aldrich (St Louis, USA). Anhydrous sodium chloride was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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7

Cultivation and Flavor Analysis of Aspergillus oryzae

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Soybeans, raw wheat flour and edible salt (NaCl) were purchased from a local supermarket (Zhenjiang, China, 2021). 3-Methylbutanal, ethanol, 3-methyl-1-butanol, ethyl propanoate, ethyl acetate, ethyl isovalerate, 2-phenylethanol, benzeneacetaldehyde, 3-(methylthio) propyl acetate, ethyl 3-(methylthio) propionate, methyl benzoate, (E)-β-damascenone, 2-phenylethyl acetate, 2-furanmethanol, 3-methylthio-1-propanol, 3-methylthio propanal, 2,6-dimethyl pyrazine, trimethyl pyrazine, 2-acetyl pyrroline, 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) and 4-hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H)-furanone (HEMF) were all ordered from Sigma-Aldrich (Shanghai, China, 2021). 3-Furaldehyde, 4-ethylphenol, 4-ethylguaiacol, 4-vinylguaiacol, 3-methylbutanoic acid, acetic acid, 2-methylbutanoic acid, dimethyl trisulfide, 1-octen-3-ol and C6-C33 n-alkanes were provided by Aladdin Holdings Group (Shanghai, China, 2021). Other analytically pure reagents were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China, 2021). A. oryzae 3.042 were obtained from the Guangdong Institute of Microbiology (Guangzhou, China, 2021).
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8

Comprehensive Volatile Compound Acquisition

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Silicon oil, 2,3-butanedione (99 %), dimethyl sulfide (DMS) (≥ 99 %), (E)-2-octenal (95 %), trans-2-hexenal (96 %), isovaleric acid (99 %) were purchased from VWR (Darmstadt, Germany). 2,3-pentanedione (≥ 96 %), dimethyl sulfone (98 %), 1-octen-3-ol (98 %), 2methyl butanal (95 %), 3-methyl butanal (97 %), heptanal (≥ 95 %), hexanal (98 %), (E,E)-2,4-nonadienal (≥ 89 %), methional (≥ 97 %), benzaldehyde (≥ 98 %), benzeneacetaldehyde (≥ 95 %), 2,4-dithiapentane (DTP) (≥ 99 %), dimethyl disulfide (DMDS) (≥ 99 %), dimethyl trisulfide (DMTS) (≥ 99 %), nonanal (≥ 95 %), 2-methyl-2-pyrroline, and alkane series standard solution (C8-C20) in hexane were purchased from Sigma-Aldrich (Taufkirchen, Germany). Short chain alkane standard mixture (C5-C8) was prepared in house; pentane, hexane, heptane (99 %) (VWR), and octane (99 %) (Sigma-Aldrich).
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