After the CD8+ T cell-mediated cytotoxicity assay, the supernatants of each well were centrifuged at 300× g for 5 min and the cell debris was removed and used for ELISA. To measure cytokine levels, anti-mouse TNF-α ELISA kits (#MTA00B; R&D Systems, Minneapolis, MN, USA) were used. All processes were performed according to the manufacturer’s instructions.
Anti mouse tnf α elisa kits
Manufactured by R&D Systems
Sourced in United States
The Anti-mouse TNF-α ELISA kits are laboratory equipment designed for the quantitative determination of mouse tumor necrosis factor alpha (TNF-α) levels in biological samples. These kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of the target analyte.
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Lab products found in correlation
2 protocols using anti mouse tnf α elisa kits
1
CD8+ T Cell Cytotoxicity Assay
2
Cytokine Quantification in Activated Microglia
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Primary cultured microglia were treated with 100 μM BzATP for different times, and the culture mediums were collected, centrifuged, and stored at − 80 °C. The concentrations of TNF-α, IL-6, IL-4 and IL-10 were measured using the flowing enzyme-linked immunoabsorbent assay (ELISA) kits, respectively, according to the manufacturers’ instructions: anti-mouse-TNF-α ELISA kits (#MTA00B, R&D Systems, USA), anti-mouse-IL-6 ELISA kits (#M6000B, R&D Systems, USA), anti-mouse-IL-4 ELISA kits (#EM003, Genetimes Technology, China) and anti-mouse-IL-10 ELISA kits (#EM005, Genetimes Technology, Shanghai, China).
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