Chromatographic grade methanol, acetonitrile, and formic acid were purchased from Fisher Scientific (FairLawn, NJ, USA). Ultrapure water was prepared using a Milli-Q purification system (Billerica, MA, USA). The gross saponins of Tribulus terrestris fruit (purity of saponins: above 60%, Lot No. 0418303) was manufactured by Changbaishan Pharmaceutical Co. Ltd. (Jilin, China).
Chromatographic grade methanol
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
Chromatographic-grade methanol is a high-purity solvent used in analytical and research laboratories for chromatographic techniques. It serves as a mobile phase component in liquid chromatography applications, providing efficient separation and detection of analytes.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (17 mentions)
Formic acid, by Thermo Fisher Scientific (8 mentions)
Heptadecanoic acid, by Merck Group (7 mentions)
Pyridine, by Maclin Biochemical Technology (7 mentions)
O methylhydroxylamine hydrochloride, by J&K Scientific (7 mentions)
Gel extraction kit, by Tiangen Biotech (1 mentions)
Methanol, by Maclin Biochemical Technology (1 mentions)
N butanol, by Maclin Biochemical Technology (1 mentions)
Ni sepharose 6 fast flow, by GE Healthcare (1 mentions)
Ethyl acetate, by Maclin Biochemical Technology (1 mentions)
Plasmid miniprep kit, by Tiangen Biotech (1 mentions)
Paeoniflorin, by Yuanye Bio-Technology (1 mentions)
Hydroxypropyl methylcellulose hpmc, by Yuanye Bio-Technology (1 mentions)
Ferulic acid, by Yuanye Bio-Technology (1 mentions)
Milli q ultrapure water system, by Merck Group (1 mentions)
Agilent 6520 q tof, by Agilent Technologies (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
Waters uplc beh c18 column, by Waters Corporation (1 mentions)
Agilent 1290 infinity uplc, by Agilent Technologies (1 mentions)
Chlorogenic acid, by Yuanye Bio-Technology (1 mentions)
25 protocols using chromatographic grade methanol
1
Tribulus terrestris Saponin Extraction
2
Optimized Protein Purification and Analysis
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DNA restriction enzymes, DNA Ligation high, Taq DNA polymerase, DNA marker, and protein marker were purchased from the Beyotime Institiute of Biotechnology (Shanghai, China). The Gel Extraction kit and Plasmid Miniprep kit were purchased from TIANGEN biotech (Beijing) Co., Ltd., (Beijing, China). Ni Sepharose™ 6 Fast Flow was purchased from GE Healthcare (Fairfield, MA, USA).
Ethyl acetate, n-butanol, and methanol were bought from Macklin (AR, Shanghai Macklin Biochemical Co. Ltd., Shanghai, China). NaCl, HCl, NaOH (AR, China National Pharmaceutical Group Corporation, Beijing, China), and distilled water were used. Chromatographic-grade methanol from Fisher (HPLC, Fisher Scientific, Waltham, MA, USA) was used for high performance liquid chromatography (HPLC) analysis. Paeoniflorin standards were purchased from YuanYe company (Shanghai, China).
Ethyl acetate, n-butanol, and methanol were bought from Macklin (AR, Shanghai Macklin Biochemical Co. Ltd., Shanghai, China). NaCl, HCl, NaOH (AR, China National Pharmaceutical Group Corporation, Beijing, China), and distilled water were used. Chromatographic-grade methanol from Fisher (HPLC, Fisher Scientific, Waltham, MA, USA) was used for high performance liquid chromatography (HPLC) analysis. Paeoniflorin standards were purchased from YuanYe company (Shanghai, China).
3
Analytical Method for Herbal Compounds
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LC-10 ATVP high performance liquid chromatography equipped with a C18 chromatographic column and SLD-10AVP UV detector (Shimadzu, Japan), DGG-9620A thermostatic blast oven (Shanghai, China), Re-52AA rotary evaporator (Shanghai, China).
Chinese materia medica were all provided by Baibao Pharmaceutical company (Gansu, China). They were authenticated by Dr. Jianying Li, School of Pharmacy Lanzhou University. Hydroxy-propyl methyl cellulose (HPMC) and pharmaceutical starch were supplied by Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). Standard samples of paeoniflorin and Ferulic acid were purchased from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). Calycosin-7-glucoside was obtained from China’s Food and Drug Verification Research Institute (Beijing, China). Chromatographic grade methanol was purchased from Fisher Scientific (Shanghai, China). Chromatographic grade acetonitrile was purchased from MREDA Technology Inc. (Beijing, China). All other reagents were analytical grade.
Chinese materia medica were all provided by Baibao Pharmaceutical company (Gansu, China). They were authenticated by Dr. Jianying Li, School of Pharmacy Lanzhou University. Hydroxy-propyl methyl cellulose (HPMC) and pharmaceutical starch were supplied by Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). Standard samples of paeoniflorin and Ferulic acid were purchased from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). Calycosin-7-glucoside was obtained from China’s Food and Drug Verification Research Institute (Beijing, China). Chromatographic grade methanol was purchased from Fisher Scientific (Shanghai, China). Chromatographic grade acetonitrile was purchased from MREDA Technology Inc. (Beijing, China). All other reagents were analytical grade.
4
Comprehensive Analysis of Phytochemicals in PM
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Chromatographic-grade methanol,
acetonitrile, and formic acid were purchased from Fisher Co. (Pittsburg,
PA). Purified water was prepared by a Milli-Q ultrapure water system
(Millipore, Bedford, MA). The dry roots of PM were purchased from
Shaanxi Jiahe Phytochem Co. Ltd. (Xi’an, Shaanxi Province,
China) and authenticated by Prof. Jianrong Li (Institute of Chinese
Materia Medica, China Academy of Chinese Medical Sciences). The standardsl -tyrosine (no. TSN-375542), l -phenylalanine (no. PYL-091102),
kynurenic acid (no. KRA-710179), chenodeoxycholic acid (no. DXC-105508),
and deoxycholic acid (no. DXC-105609) were purchased from Stanford
Chemicals (Lake Forest, CA).
acetonitrile, and formic acid were purchased from Fisher Co. (Pittsburg,
PA). Purified water was prepared by a Milli-Q ultrapure water system
(Millipore, Bedford, MA). The dry roots of PM were purchased from
Shaanxi Jiahe Phytochem Co. Ltd. (Xi’an, Shaanxi Province,
China) and authenticated by Prof. Jianrong Li (Institute of Chinese
Materia Medica, China Academy of Chinese Medical Sciences). The standards
kynurenic acid (no. KRA-710179), chenodeoxycholic acid (no. DXC-105508),
and deoxycholic acid (no. DXC-105609) were purchased from Stanford
Chemicals (Lake Forest, CA).
5
Identification of Bioactive Compounds in Plant Extracts
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Periplocin (≥98%), periplocymarin (≥98%), periplogenin (≥98%), and scopoletin (≥98%) were purchased from Chengdu Durst Biotechnology Co., Ltd. (Chengdu, China). Isovanillin (≥98%), isovanillic acid (≥98%), chlorogenic acid (≥98%), ursolic acid (≥98%), and oleanolic acid (≥98%) were obtained from Shanghai Yuanye biotechnology Co., Ltd. (Shanghai, China). Chromatographic grade methanol and acetonitrile were purchased from Fisher company (Thermo Fisher Scientific (China) Co. Ltd., Shanghai, China). Anhydrous ethanol (chromatographically pure) was obtained from Concord technologies Ltd. (Tianjin, China). Water was obtained from a Milli-Q purification system (Millipore, Bedford, MA, USA). Nylon membranes were purchased from Tianjin Bojin Technology Co., Ltd. (Tianjin, China).
The UPLC-Q-TOF-MS/MS system is comprised of an Agilent 1290 Infinity UPLC (Agilent Technologies Inc., Palo Alto, CA, USA) and an Agilent 6520 QTOF. The UPLC system includes an Agilent 4220 binary pump combined with a degasser, an Agilent 4212 diode array detector, an Agilent 4226 well plate sampler, and an Agilent 1316 thermostatic column compartment. Chromatographic separation was performed on a Waters UPLC®BEH C18 column (2.1 × 100 mm, 1.7 µm, Waters, Milford, MA, USA).
The UPLC-Q-TOF-MS/MS system is comprised of an Agilent 1290 Infinity UPLC (Agilent Technologies Inc., Palo Alto, CA, USA) and an Agilent 6520 QTOF. The UPLC system includes an Agilent 4220 binary pump combined with a degasser, an Agilent 4212 diode array detector, an Agilent 4226 well plate sampler, and an Agilent 1316 thermostatic column compartment. Chromatographic separation was performed on a Waters UPLC®BEH C18 column (2.1 × 100 mm, 1.7 µm, Waters, Milford, MA, USA).
6
Standardized PLGA Nanoparticle Preparation
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The AP sample (purity ≥98%) was a gift from the Shandong Academy of Medical Sciences (Jinan, People’s Republic of China). The PUE standard (Batch Number 110752-200912) was acquired from the National Institutes for Food and Drug Control (Beijing, People’s Republic of China). PLGA (lactide/glycolide =50:50, molecular weight =10,000 Da) was obtained from Jinan Daigang Biomaterial Co., Ltd. (Jinan, People’s Republic of China). Polysorbate 80 (Tween 80) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China). Chromatographic-grade methanol and acetonitrile were obtained from Fisher Scientific (Fair Lawn, NJ, USA). The dialysis membrane was purchased from Viskase Company, Inc. (Darien, IL, USA). All the other chemicals and solvents used in this study were of analytical grade.
Wistar rats were supplied by the Laboratory Animal Center of Shandong University (Jinan, People’s Republic of China).
Wistar rats were supplied by the Laboratory Animal Center of Shandong University (Jinan, People’s Republic of China).
7
Semipreparative HPLC and Spectroscopic Analysis of Ginseng Compounds
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Semipreparative high performance liquid chromatography was carried out on an Agela Innoval ODS-2 column (5 μm, 10 × 250 nm, China) with an LC3000 instrument (Beijing Tong Heng Innovation Technology Co., Ltd. China). NMR spectra: Bruker ARX-600 spectrometer in pyridine-d5 with trimethylchlorosilane as internal standard.
The flash extractor was obtained by All Herbal Scientech, LLC (JHBE-20A, Beijing, China). HPLC-FT-ICR-MS was performed with auto-sampler, thermostatic column chamber, online degasser, quadruple pump on Agilent 1260 system and diode-array detector (Agilent Corporation, Santa Clara, CA, USA) with ESI interface and a 7.0 tesla magnet. HPLC analyses were performed on CXTH LC-3000 (Beijing, China). Octadecyl silica chromatographic column [Agilent 5 HC-C18 column (250 × 4.6 mm)]. Chromatographic grade methanol, acetonitrile and purified water were purchased from Fisher Scientific International Inc.and Wahaha (Hangzhou, China) respectively.
Samples of MCG and CG were collected from Huanren, Liaoning Province, China. All of the plants were identified as 15 and 5 years ginseng by Prof. Jincai Lu of Shenyang Pharmaceutical University. A voucher specimen (PT 20190316) was deposited in the herbarium of Shenyang Pharmaceutical University. The samples were dried at 45 °C to constant weight.
The flash extractor was obtained by All Herbal Scientech, LLC (JHBE-20A, Beijing, China). HPLC-FT-ICR-MS was performed with auto-sampler, thermostatic column chamber, online degasser, quadruple pump on Agilent 1260 system and diode-array detector (Agilent Corporation, Santa Clara, CA, USA) with ESI interface and a 7.0 tesla magnet. HPLC analyses were performed on CXTH LC-3000 (Beijing, China). Octadecyl silica chromatographic column [Agilent 5 HC-C18 column (250 × 4.6 mm)]. Chromatographic grade methanol, acetonitrile and purified water were purchased from Fisher Scientific International Inc.and Wahaha (Hangzhou, China) respectively.
Samples of MCG and CG were collected from Huanren, Liaoning Province, China. All of the plants were identified as 15 and 5 years ginseng by Prof. Jincai Lu of Shenyang Pharmaceutical University. A voucher specimen (PT 20190316) was deposited in the herbarium of Shenyang Pharmaceutical University. The samples were dried at 45 °C to constant weight.
8
Antioxidant Biomarker Analysis of Pharmacological Agents
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1-Acetyl-2-phenylhydrazine (APH) was purchased from Shanghai Aladdin industrial Co., Ltd. (batch number: 201406, Shanghai, China). Cyclophosphamide (CTX) was purchased from Jiangsu Hengrui Medicine Co., Ltd. (batch number: 18062625). Positive control drug, Asini Corii Colla (ACC), was purchased from Shandong Donge Co., Ltd. (batch number: 1709003).
The levels of SOD (A001–1-2, Nanjing Jiancheng Bioengineering Institute, China), GSH (C011–2, Suzhou Kaerwen Bioengineering Institute), GSH-Px (C006–2-1, Nanjing Jiancheng Bioengineering Institute), and MDA (C003–1-2, Suzhou Kaerwen Bioengineering Institute) in serum and ATP (202,005, Suzhou Kaerwen Bioengineering Institute) in the liver were measured using specific kits, in accordance with the manufacturer’s instructions. All assays were performed in triplicate.
Chromatographic -grade methanol and acetonitrile were purchased from Fisher Scientific (Bridgewater, NJ, USA). Ultrapure water was produced by a Milli-Q water purification system (Millipore, Bedford, MA, USA), and formic acid (LC/MS grade) was purchased from Fisher Scientific (Bridgewater, NJ, USA).
The levels of SOD (A001–1-2, Nanjing Jiancheng Bioengineering Institute, China), GSH (C011–2, Suzhou Kaerwen Bioengineering Institute), GSH-Px (C006–2-1, Nanjing Jiancheng Bioengineering Institute), and MDA (C003–1-2, Suzhou Kaerwen Bioengineering Institute) in serum and ATP (202,005, Suzhou Kaerwen Bioengineering Institute) in the liver were measured using specific kits, in accordance with the manufacturer’s instructions. All assays were performed in triplicate.
Chromatographic -grade methanol and acetonitrile were purchased from Fisher Scientific (Bridgewater, NJ, USA). Ultrapure water was produced by a Milli-Q water purification system (Millipore, Bedford, MA, USA), and formic acid (LC/MS grade) was purchased from Fisher Scientific (Bridgewater, NJ, USA).
9
Ginseng Phytochemical Fingerprinting Protocol
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A KQ3200DB ultrasonic cleaning bath (Kunshan Ultrasonic Instrument Co., Ltd., Shanghai, China) was used. Chromatographic grade methanol and acetonitrile were purchased from Fisher Scientific International Inc., (Hampton, NH, USA). Purified water was purchased from Wahaha (Hangzhou, China).
The ginseng sample (Huanren City, Liaoning Province) was identified as 15-year-old ginseng by Professor Lu Jincai of Shenyang Pharmaceutical University. A voucher specimen (PT 20200411) was kept in the herbarium of Shenyang Pharmaceutical University. The samples were dried at 45 °C to constant weight. Ginsenoside Rg1, Re, Rh1, Rb1, RC, F1, RD, Rg3, Rg5, Rh2, PPT, and PPD were self-made in the laboratory (purity > 98%).
The ginseng sample (Huanren City, Liaoning Province) was identified as 15-year-old ginseng by Professor Lu Jincai of Shenyang Pharmaceutical University. A voucher specimen (PT 20200411) was kept in the herbarium of Shenyang Pharmaceutical University. The samples were dried at 45 °C to constant weight. Ginsenoside Rg1, Re, Rh1, Rb1, RC, F1, RD, Rg3, Rg5, Rh2, PPT, and PPD were self-made in the laboratory (purity > 98%).
10
Metabolomic Analysis of LPS-Treated Cells
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LPS (Escherichia coli serotype0111:B4), heptadecanoic acid (purity ≥98%), an internal standard (IS), and N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (BSTFA+1% TMCS; v/v) were purchased from Sigma–Aldrich (Saint Louis, MO, USA). O-Methylhydroxylamine hydrochloride (98.0% purity) was obtained from J&K Scientific Ltd (Beijing, China). Pyridine was obtained from Shanghai Macklin Biochemical (Shanghai, China). Chromatographic-grade methanol was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Water was purchased from Hangzhou Wahaha Company (Hangzhou, China).
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