Thermofinnigan lcq duo

HPLC–PDA-MS was used to identify the phytoconstituents of ETME using ThermoFinnigan LCQ-Duo ion trap mass spectrometer (ThermoElectron Corporation, Waltham, MA, USA) with an ESI source (ThermoQuest Corporation, Austin, TX, USA)^{25 (link),26 (link)}. Details of the HPLC system are described in the supplementary file.

A ThermoFinnigan LCQ-Duo ion trap mass spectrometer (ThermoElectron Corporation, Waltham, MA, USA) with an ESI source (ThermoQuest Corporation, Austin, TX, USA) was used to identify the phytochemical composition of the leaf extract as previously reported by Ghareeb et al. [5 (link)].

A ThermoFinnigan LCQ-Duo ion trap mass spectrometer (ThermoElectron Corporation, Waltham, MA, USA) with an ESI source (ThermoQuest Corporation, Austin, TX, USA) was used. A Discovery HS F5 column (15 cm × 4.6 mm ID, 5 µm particles) (Sigma-Aldrich Co, Steinheim, Germany) was utilized with the ThermoFinnigan HPLC system. The mobile phase was water and acetonitrile (ACN) (Sigma-Aldrich GmbH, Steinheim, Germany) (0.1% formic acid each). At 0 min, ACN was 5%, then increased to 30% over 60 min, and finally to 90% within the last 30 min. The flow rate was kept at 1 mL/min with a 1:1 split before the ESI source. The MS operated in the negative mode as previously reported [39 (link)].

The extracts were analyzed utilizing a ThermoFinnigan LCQ-Duo ion trap mass spectrometer (ThermoElectron Corporation, Waltham, Ma, USA) with an ESI source (ThermoQuest Corporation, Austin, Tx, USA). A ThermoFinnigan HPLC system using a C18 reversed-phase column (Zorbax Eclipse XDB-C18, Rapid resolution, 4.6 × 150 mm, 3.5 µm, Agilent, Santa Clara, CA, USA) was used^{17 (link)}. Water and acetonitrile (ACN) (Sigma-Aldrich GmbH, Germany) (0.1% formic acid each) were used as a mobile phase. At 0 min, ACN was 5% and then increased to 30% in 60 min at 1 mL/min with a 1:1 split before the ESI source. Autosampler surveyor ThermoQuest was utilized to inject the extracts and the system was controlled by Xcalibur software (Xcalibur^TM 2.0.7, Thermo Fischer Scientific, Waltham, Ma, USA). The MS operated in the negative mode as reported before^{18 (link)}. The ions were detected in a full scan mode and mass range of 50–2000 m/z.